CAJ | 학술논문

为进一步研究肉羊性控精液在胚胎生产上的应用，以萨福克、无角陶赛特纯种肉用绵羊为供体，本地小尾寒羊杂交后代和湖羊为受体，利用子宫角深部输精及一般人工授精等辅助生殖技术使用性控冻精与普通鲜精对两品种肉羊进行人工授精，统计不同品种肉羊的受胎率和可用胚数，对获得的性控胚胎和普通胚胎进行移植，并对比其移植效果。结果表明：萨福克肉羊和无角陶赛特肉羊超排后黄体数差异不明显（P〉0．05）；普通精液榆精所获得的胚胎数板显著高于性控冻精（P〈0．01）；在受胎率上也做了比较，但差异不显著，受胎率43．2％。由于精液在通过流式细胞仪时，对精子顶体造成一定损伤，因此，无论是子宫角榆精或阴道子宫颈输精均影响受精率，但性控精液的推广应用确实可以加速家畜遗传育种速度，缩短育种周期。鉴于此，今后对性控精液的研究重点应该放在精液分离过程中对精子的保护，以减少对精子的损伤。
위진일보연구육양성공정액재배태생산상적응용，이살복극、무각도새특순충육용면양위공체，본지소미한양잡교후대화호양위수체，이용자궁각심부수정급일반인공수정등보조생식기술사용성공동정여보통선정대량품충육양진행인공수정，통계불동품충육양적수태솔화가용배수，대획득적성공배태화보통배태진행이식，병대비기이식효과。결과표명：살복극육양화무각도새특육양초배후황체수차이불명현（P〉0．05）；보통정액유정소획득적배태수판현저고우성공동정（P〈0．01）；재수태솔상야주료비교，단차이불현저，수태솔43．2％。유우정액재통과류식세포의시，대정자정체조성일정손상，인차，무론시자궁각유정혹음도자궁경수정균영향수정솔，단성공정액적추엄응용학실가이가속가축유전육충속도，축단육충주기。감우차，금후대성공정액적연구중점응해방재정액분리과정중대정자적보호，이감소대정자적손상。
The donors were Suffolk and Poll Dorset, recipients were Small Tail Han Sheep and Hu Sheep. The frozen sexing semen and normal fresh semen were used in insemination of donors. The fertilization rate and available embryos number of these two kinds of semen were compared in order to provide reference for the further application of frozen sexing semen in embryo production. Endoscope insemination and normal artificial insemination of those two kinds of semen were applied in embryo production of two mutton sheep breeds;the fertilization rate and available embryos number of those two mutton sheep breeds were compared,and the transfer effect of sexing embryos and normal embryos were compared as well. The results showed that the number of corpus luteum of those two breeds after superovulation showed no difference （P〉0. 05）. The available embryos number from normal fresh semen was several significant higher than that from frozen sexing semen （P〈0. 01）. The pregnancy rate for the recipients was 43.2 % and showed no difference. However, the frozen sexing semen negatively affected fertilization rate of donors because of the sperm acrosome damage after the sperm processing in flow cytometry, the application of sexing semen could improve breeding process. Therefore,in the future research,the hot topic would be how to protect sperm while applying the X/Y sperm selection.