黑龙江农业科学
黑龍江農業科學
흑룡강농업과학
HEILONGJINAG AGRICULTURAL SCIENCE
2012年
4期
23-27
,共5页
曹士亮%史桂荣%王成波%贺强%蔡泉%卢翠华%林忠平
曹士亮%史桂榮%王成波%賀彊%蔡泉%盧翠華%林忠平
조사량%사계영%왕성파%하강%채천%로취화%림충평
转基因玉米%苗期%耐盐性
轉基因玉米%苗期%耐鹽性
전기인옥미%묘기%내염성
transgenic maize%seedling stage%salt resistance
为了明确外源基因提高玉米耐盐性的效果,以6份转BcWRKYI基因的玉米杂交组合及其对应的6份非转基因对照组合为试材,对其发芽率、耐盐级别、株高、干重、脯氨酸含量等7个指标进行分析。结果表明:耐盐级别、发芽率、干重、叶绿素舍量和相对电导率的变化均达到显著水平,株高的变化达到极显著水平,脯氨酸含量转基因与非转基因组合间差异不显著。
為瞭明確外源基因提高玉米耐鹽性的效果,以6份轉BcWRKYI基因的玉米雜交組閤及其對應的6份非轉基因對照組閤為試材,對其髮芽率、耐鹽級彆、株高、榦重、脯氨痠含量等7箇指標進行分析。結果錶明:耐鹽級彆、髮芽率、榦重、葉綠素捨量和相對電導率的變化均達到顯著水平,株高的變化達到極顯著水平,脯氨痠含量轉基因與非轉基因組閤間差異不顯著。
위료명학외원기인제고옥미내염성적효과,이6빈전BcWRKYI기인적옥미잡교조합급기대응적6빈비전기인대조조합위시재,대기발아솔、내염급별、주고、간중、포안산함량등7개지표진행분석。결과표명:내염급별、발아솔、간중、협록소사량화상대전도솔적변화균체도현저수평,주고적변화체도겁현저수평,포안산함량전기인여비전기인조합간차이불현저。
In order to analyze the effect of salt tolerance improvement by exogenous gene transformation, six transgenic maize crosses with BcWRKYI gene and six respective non-transgenic control of them were used to identify salt toleranc in seedling stage. Seven indicators such as the germination rate, salt resistance level, plant height,dry weight,proline content and so on were analyzed to evaluate the effect of the transformation of Bc- WRKYI gene on improving the salt resistance. The results indicated that salt resistance level,germination rate, dry weight,chlorophyll content, relative electric conductivity appeared significant differences, plant height appeared extremely significant differences,however,the difference of proline content was not significant.