实用医学杂志
實用醫學雜誌
실용의학잡지
THE JOURNAL OF PRACTICAL MEDICINE
2014年
7期
1066-1069
,共4页
人参皂甙Rg3%K562细胞%P27%增殖
人參皂甙Rg3%K562細胞%P27%增殖
인삼조대Rg3%K562세포%P27%증식
Ginsenoside Rg3%K562 cell%P27%Proliferation
目的：探讨人参皂甙单体Rg3诱导P27表达变化对人红白血病细胞株K562细胞增殖的影响。方法：将人红白血病细胞株K562细胞传代培养至对数生长期，分组加入不同浓度（6．25、12．5、25、50、100μg/mL）的人参皂甙单体Rg3，并设置空白对照组（0μg/mL Rg3）。培养3 d后，瑞氏-姬姆萨染色后，显微镜观察K562细胞生长情况，四氮唑蓝（MTT）比色法测定 K562细胞增殖活性，荧光定量 RT-PCR 法检测 P27 mRNA 的表达。结果：不同浓度（0、6．25、12．5、25、50、100μg/mL）人参皂甙单体 Rg3作用于 K562细胞后， MTT比色法测定显示，Rg3组增殖抑制率逐渐升高，与空白对照组相比具有明显差异（P＜0．05）；荧光定量RT-PCR检测结果显示较高浓度（25、50、100μg/mL）人参皂甙Rg3组P27 mRNA表达水平比空白对照组明显升高，差异有显著性（P＜0．05）。结论：人参皂甙单体Rg3能通过诱导P27表达升高抑制K562细胞的增殖。
目的：探討人參皂甙單體Rg3誘導P27錶達變化對人紅白血病細胞株K562細胞增殖的影響。方法：將人紅白血病細胞株K562細胞傳代培養至對數生長期，分組加入不同濃度（6．25、12．5、25、50、100μg/mL）的人參皂甙單體Rg3，併設置空白對照組（0μg/mL Rg3）。培養3 d後，瑞氏-姬姆薩染色後，顯微鏡觀察K562細胞生長情況，四氮唑藍（MTT）比色法測定 K562細胞增殖活性，熒光定量 RT-PCR 法檢測 P27 mRNA 的錶達。結果：不同濃度（0、6．25、12．5、25、50、100μg/mL）人參皂甙單體 Rg3作用于 K562細胞後， MTT比色法測定顯示，Rg3組增殖抑製率逐漸升高，與空白對照組相比具有明顯差異（P＜0．05）；熒光定量RT-PCR檢測結果顯示較高濃度（25、50、100μg/mL）人參皂甙Rg3組P27 mRNA錶達水平比空白對照組明顯升高，差異有顯著性（P＜0．05）。結論：人參皂甙單體Rg3能通過誘導P27錶達升高抑製K562細胞的增殖。
목적：탐토인삼조대단체Rg3유도P27표체변화대인홍백혈병세포주K562세포증식적영향。방법：장인홍백혈병세포주K562세포전대배양지대수생장기，분조가입불동농도（6．25、12．5、25、50、100μg/mL）적인삼조대단체Rg3，병설치공백대조조（0μg/mL Rg3）。배양3 d후，서씨-희모살염색후，현미경관찰K562세포생장정황，사담서람（MTT）비색법측정 K562세포증식활성，형광정량 RT-PCR 법검측 P27 mRNA 적표체。결과：불동농도（0、6．25、12．5、25、50、100μg/mL）인삼조대단체 Rg3작용우 K562세포후， MTT비색법측정현시，Rg3조증식억제솔축점승고，여공백대조조상비구유명현차이（P＜0．05）；형광정량RT-PCR검측결과현시교고농도（25、50、100μg/mL）인삼조대Rg3조P27 mRNA표체수평비공백대조조명현승고，차이유현저성（P＜0．05）。결론：인삼조대단체Rg3능통과유도P27표체승고억제K562세포적증식。
Objective To explore the effects of ginsenoside Rg3 on the expression of P27 in human erythrol-eukemia cell line K562 and cell proliferation. Methods Human erythroleukemia cell line K562 cells were cultured to exponential phase, then K562 cells were treated with different concentrations of Rg3 (6.25, 12.5, 25, 50, and 100 μg/mL) as Rg3 group, and cells treated without Rg3 (0 μg/mL) were take as control group. After 3 days, K562 cells were observed by Wright-Giemsa staining with microscopy , the proliferation of K562 cells were examined by tetrazolium salt (MTT) assay, and the expression of P27 mRNA were detected by fluorescent quantitative RT-PCR assay. Results MTT assay showed that after treatment with Rg3,the inhibition rate (IR) of proliferation of cells in Rg3 groups were increased gradually , and the differences were significant compared with the control group (P < 0.05). The results of fluorescent quantitative RT-PCR showed the levels of P27 mRNA expression in 25,50 and 100 μg/mL Rg3 groups were significant higher than that of control group (P < 0.05). Conclusion The ginsenoside Rg3 can inhibit the proliferation of K562 cells by inducing the expression of P27.
