大连医科大学学报
大連醫科大學學報
대련의과대학학보
JOURNAL OF DALIAN MEDICAL UNIVERSITY
2014年
2期
115-119
,共5页
维甲酸%舌肌发育%生肌调控因子%腭裂
維甲痠%舌肌髮育%生肌調控因子%腭裂
유갑산%설기발육%생기조공인자%악렬
retinoic acid%tongue myogenesis%myogenic regulatory factors%cleft palate
目的：通过检测生肌决定因子基因家族成员Myf5和MyoD在维甲酸诱导腭裂小鼠舌肌发育中的表达，探究维甲酸是否在舌肌发育早期影响其肌向分化。方法建立维甲酸诱导腭裂小鼠模型，利用免疫组化染色法和实时定量PCR法，检测胚胎13天即舌肌发育早期实验组和对照组小鼠舌肌中Myf5和MyoD的分布和表达水平。结果免疫组化结果显示Myf5和MyoD在舌肌细胞的细胞核中表达，实验组Myf5和MyoD的蛋白表达均低于对照组，且差异有显著性意义（P＜0．01）。实时定量PCR结果显示实验组Myf5和MyoD的mRNA表达水平均低于对照组，且差异有显著性意义（ P ＜0．01）。结论 Myf5和MyoD的异常低表达提示了维甲酸可能直接导致舌肌发育和分化的延迟，进而导致腭裂的发生或使其表型加重。
目的：通過檢測生肌決定因子基因傢族成員Myf5和MyoD在維甲痠誘導腭裂小鼠舌肌髮育中的錶達，探究維甲痠是否在舌肌髮育早期影響其肌嚮分化。方法建立維甲痠誘導腭裂小鼠模型，利用免疫組化染色法和實時定量PCR法，檢測胚胎13天即舌肌髮育早期實驗組和對照組小鼠舌肌中Myf5和MyoD的分佈和錶達水平。結果免疫組化結果顯示Myf5和MyoD在舌肌細胞的細胞覈中錶達，實驗組Myf5和MyoD的蛋白錶達均低于對照組，且差異有顯著性意義（P＜0．01）。實時定量PCR結果顯示實驗組Myf5和MyoD的mRNA錶達水平均低于對照組，且差異有顯著性意義（ P ＜0．01）。結論 Myf5和MyoD的異常低錶達提示瞭維甲痠可能直接導緻舌肌髮育和分化的延遲，進而導緻腭裂的髮生或使其錶型加重。
목적：통과검측생기결정인자기인가족성원Myf5화MyoD재유갑산유도악렬소서설기발육중적표체，탐구유갑산시부재설기발육조기영향기기향분화。방법건립유갑산유도악렬소서모형，이용면역조화염색법화실시정량PCR법，검측배태13천즉설기발육조기실험조화대조조소서설기중Myf5화MyoD적분포화표체수평。결과면역조화결과현시Myf5화MyoD재설기세포적세포핵중표체，실험조Myf5화MyoD적단백표체균저우대조조，차차이유현저성의의（P＜0．01）。실시정량PCR결과현시실험조Myf5화MyoD적mRNA표체수평균저우대조조，차차이유현저성의의（ P ＜0．01）。결론 Myf5화MyoD적이상저표체제시료유갑산가능직접도치설기발육화분화적연지，진이도치악렬적발생혹사기표형가중。
Objective To investigate the expressions of the myogenic regulation factors ( MRFs) Myf5 and MyoD in tongue myogenesis of cleft palate mice induced by RA , and to explore whether the retinoic acid ( RA) affect the myogenic differen-tiation of tongue in early development stage .Meth ods Mouse model of RA-induced cleft palate was set up .Immunohisto-chemistry and real-time PCR were used to detect the distribution and expression levels of Myf 5 and MyoD in tongue of RA-induced cleft palate group and wild type at embryonic day 13 (E13), the early stage of tongue myogenesis .Results Immu-nohistochemical results showed that Myf 5 and MyoD expressed in the nucleus of tongue muscle cells , and the protein levels of them were down regulated in RA -treated group (P<0.01).Real-time PCR results showed that the mRNA expression level of Myf5 and MyoD also decreased significantly in RA -induced cleft palate group at E 13 compared to the wild type (P<0.01).Conclusion The aberrant low expression of Myf5 and MyoD suggests that the delay development and differen -tiation of tongue muscle may be induced directly by RA .And this could lead to cleft palate or exacerbate its phenotype of cleft palate .
