CAJ | 학술논문

目的：调查原发性胆汁性肝硬化（PBC）、自身免疫性肝炎（AIH）及AIH-PBC重叠综合征患者外周血淋巴细胞亚群频率变化及其临床意义。方法本中心2001年6月-2010年12月期间，对41例AIH-PBC、37例AIH和36例PBC患者，以及50例健康人群外周血进行淋巴细胞亚群频率检测。分析患者年龄、性别、肝功能、是否肝硬化及淋巴细胞亚群频率的变化。结果与健康组相比，AIH-PBC重叠综合征组、PBC组和AIH组的CD3＋CD4＋T细胞频率显著升高，而CD3-CD16＋CD56＋NK细胞频率显著降低；PBC组和AIH-PBC重叠综合征中CD4＋/CD8＋比值、CD3-CD19＋B细胞频率偏高，CD3＋CD8＋％T细胞频率降低。在疾病发展的不同阶段，AIH-PBC重叠综合征组和PBC组中，肝硬化组CD3＋％T细胞频率、CD3＋CD8＋％T细胞频率较非肝硬化组偏低。结论通过回顾性分析健康人群和AIH、PBC及AIH-PBC重叠综合征患者淋巴细胞亚群的分布特点及其与疾病进展的关系，为临床科学评价上述自身免疫性肝病人群的免疫状态提供重要的免疫指标。
목적：조사원발성담즙성간경화（PBC）、자신면역성간염（AIH）급AIH-PBC중첩종합정환자외주혈림파세포아군빈솔변화급기림상의의。방법본중심2001년6월-2010년12월기간，대41례AIH-PBC、37례AIH화36례PBC환자，이급50례건강인군외주혈진행림파세포아군빈솔검측。분석환자년령、성별、간공능、시부간경화급림파세포아군빈솔적변화。결과여건강조상비，AIH-PBC중첩종합정조、PBC조화AIH조적CD3＋CD4＋T세포빈솔현저승고，이CD3-CD16＋CD56＋NK세포빈솔현저강저；PBC조화AIH-PBC중첩종합정중CD4＋/CD8＋비치、CD3-CD19＋B세포빈솔편고，CD3＋CD8＋％T세포빈솔강저。재질병발전적불동계단，AIH-PBC중첩종합정조화PBC조중，간경화조CD3＋％T세포빈솔、CD3＋CD8＋％T세포빈솔교비간경화조편저。결론통과회고성분석건강인군화AIH、PBC급AIH-PBC중첩종합정환자림파세포아군적분포특점급기여질병진전적관계，위림상과학평개상술자신면역성간병인군적면역상태제공중요적면역지표。
Objective To investigate the changes in frequencies of peripheral blood lymphocyte (PBL)subsets and their clinical signifi-cance in patients with primary biliary cirrhosis (PBC),autoimmune hepatitis (AIH),and AIH-PBC overlap syndrome.Methods Forty-one patients with AIH-PBC overlap syndrome,37 patients with AIH,36 patients with PBC,and 50 healthy persons were included in the retrospective study.The frequencies of PBL subsets were determined by flow cytometry.The changes in frequencies of PBL subsets and their clinical significance were investigated.Results Compared with the healthy control group,the AIH-PBC group,PBC group,and AIH group had a significantly increased frequency of CD3 +CD4 +T cells and a significantly decreased frequency of CD3 -CD16 +CD56 +NK cells;the PBC group and AIH-PBC group had significantly increased CD4 +/CD8 +ratio and frequency of CD3 -CD19 +B cells and a sig-nificantly decreased frequency of CD3 +CD8 +T cells.During different stages of diseases,in the AIH-PBC group and PBC group,the fre-quencies of CD3 +T cells and CD3 +CD8 +T cells were significantly lower in patients with liver cirrhosis than in those without liver cirrho-sis.Conclusion The changes in frequencies of PBL subsets are related to the progression of autoimmune liver diseases,providing important immunological parameters for clinical evaluation of the immune status in patients with autoimmune liver diseases.