CAJ | 학술논문

转录因子TRRAP是维系生命体正常活动的重要蛋白，目前对其功能作用还知之甚少，相关研究方兴未艾．2011年发现TRRAP一个多发点突变与肿瘤相关，该突变亟须在人群样品中进行检测，以增加对该分子与肿瘤发生关系的了解．本文设计了一个通过PCR扩增，然后酶切的检测方法，有助于了解肿瘤的发生机理．
전록인자TRRAP시유계생명체정상활동적중요단백，목전대기공능작용환지지심소，상관연구방흥미애．2011년발현TRRAP일개다발점돌변여종류상관，해돌변극수재인군양품중진행검측，이증가대해분자여종류발생관계적료해．본문설계료일개통과PCR확증，연후매절적검측방법，유조우료해종류적발생궤리．
Transformation/ Transcription Domain-associated Protein; TRRAP, an important transcription factor, was found in 1998 by McMahon, it is still not very clear about its function and its company proteins to date. 2011, Wei found a recurrent point mutation in conserved site of TRRAP by exome sequencing. Here was a detect method for this specific point mutation by PCR and nuclear restriction enzyme digest, through detecting the re- currence mutation situation on tumor patients samples, we will get more comprehensions for this important transcription factor.