CAJ | 학술논문

本试验旨在研究植物甾醇对雌性小鼠生长及生殖激素的影响。选用50只35日龄雌性小鼠，适应1周后随机分为5组，分别为：对照组、植物油组（给予0.1 mL植物油）及植物甾醇低、中、高三个剂量组（每日灌胃20、80和320 mg/kg植物甾醇）。连续灌胃3周，第1天和第22天称取小鼠体重，然后眼球采血，制备血清，对小鼠血清中的雌二醇、孕酮、催乳素水平进行检测，研究不同添加量的植物甾醇对小鼠生长及生殖激素的影响。结果显示：与对照组相比，植物油组小鼠体增重差异显著（P＜0.05）。与植物油组相比，植物甾醇处理组小鼠体增重先升高后降低，但差异不显著（P＞0.05）。植物甾醇对小鼠血清E2水平有不同程度的提高，其中中剂量组小鼠的雌二醇水平最高（270.52±18.10 pmol/L）；随着植物甾醇灌胃剂量的增加，小鼠孕酮水平都有不同程度的下降，其中植物甾醇中剂量组小鼠血清孕酮水平显著低于对照组（P＜0.05）。植物甾醇灌胃组小鼠催乳素水平与对照组相比，差异不显著（P＞0.05）。表明低剂量植物甾醇可提高KM雌性小鼠的体增重；植物甾醇能提高KM雌性小鼠血清雌二醇水平，但对孕酮和催乳素的作用不显著。
본시험지재연구식물치순대자성소서생장급생식격소적영향。선용50지35일령자성소서，괄응1주후수궤분위5조，분별위：대조조、식물유조（급여0.1 mL식물유）급식물치순저、중、고삼개제량조（매일관위20、80화320 mg/kg식물치순）。련속관위3주，제1천화제22천칭취소서체중，연후안구채혈，제비혈청，대소서혈청중적자이순、잉동、최유소수평진행검측，연구불동첨가량적식물치순대소서생장급생식격소적영향。결과현시：여대조조상비，식물유조소서체증중차이현저（P＜0.05）。여식물유조상비，식물치순처리조소서체증중선승고후강저，단차이불현저（P＞0.05）。식물치순대소서혈청E2수평유불동정도적제고，기중중제량조소서적자이순수평최고（270.52±18.10 pmol/L）；수착식물치순관위제량적증가，소서잉동수평도유불동정도적하강，기중식물치순중제량조소서혈청잉동수평현저저우대조조（P＜0.05）。식물치순관위조소서최유소수평여대조조상비，차이불현저（P＞0.05）。표명저제량식물치순가제고KM자성소서적체증중；식물치순능제고KM자성소서혈청자이순수평，단대잉동화최유소적작용불현저。
The experiment was conducted to study the effects of phytosterol on growth and repro-ductive hormone in female mice. A total of 50 35-day-old female mice are adapted for one week and randomly divided into 5 groups respectively: control group (0.1 mL vegetable oil), phytosterol low, medium, high of three dose groups(daily intragastric 20, 80, 320 mg/kg phytosterol). After three weeks of Intragastric administration, body weight of mice at the first day and the twen-ty-second day were recorded, then blood from the eyeball were collected to prepare serum, serum levels of estradiol, progesterone, prolactin were detected. The influence of different levels of phytosterol on the growth and reproductive hormone in mice were analyzed. The results showed that:Compared with the control group, the body weight gain of vegetable group were significantly differ-ent(P<0.05). Compared with the vegetable group, body weight gain of phytosterols treatment group had first increased and then decreased, the difference between them was not significant(P>0.05). Phytosterol could improve the serum levels of E on different degrees2, medium dose group had the highest estradiol levels(270.52 ± 18.10 pmol/L). With the increase ofthe doses of phytosterols ga-vage, progesterone levels of mice declined in different degrees.The serum progesterone levels of phytosterol medium dose group was significant lower than the control group(P<0.05). Compared with the control group, Prolactin levels of phytosterols mice group had no significant difference(P>0.05). Low dose phytosterol can improve the body weight gain of KM female mice, Phytosterol can im-prove serum estradiol levels of KM female mice, but the level of progesterone and prolactin were not significant.