河北医学
河北醫學
하북의학
HEBEI MEDICINE
2014年
6期
901-904
,共4页
袁明勇%张仲林%王丹%赵霞%廖昌军%郑玲利
袁明勇%張仲林%王丹%趙霞%廖昌軍%鄭玲利
원명용%장중림%왕단%조하%료창군%정령리
抗氧化活性%醇提浸膏%总多糖%水提醇沉
抗氧化活性%醇提浸膏%總多糖%水提醇沉
항양화활성%순제침고%총다당%수제순침
Antioxidant activity%Ethanol extraction%Total polysaccharide%Water extraction and alcohol precipitation
目的：筛选玉屏风散具有抗氧化活性的有效部位，为药剂学以及药理学的研究奠定基础。方法：采用水提醇沉法提取玉屏风散中的粗多糖，用Sevag法除蛋白得到精制多糖，另外用乙醇回流提取药材粉末后用旋转蒸发仪蒸干得到醇提浓缩浸膏，然后分别溶解在去离子水中，用四种不同极性的溶剂萃取，得到四种不同极性部位的浓缩物，将总多糖与四种不同极性溶剂的萃取物分别用邻苯三酚自氧化法分别测定其抗氧化活性。结果：正丁醇层具有较高的超氧阴离子自由基清除率，乙酸乙酯层的清除率次之，玉屏风散水提液的超氧阴离子自由基清除率略高于石油醚层与二氯甲烷层。结论：玉屏风散具有较强的超氧阴离子自由基清除能力的活性物质的混合物，其极性可能比较大，易被正丁醇和乙酸乙酯萃取出来，而被非极性的石油醚和二氯甲烷萃取的量很少。
目的：篩選玉屏風散具有抗氧化活性的有效部位，為藥劑學以及藥理學的研究奠定基礎。方法：採用水提醇沉法提取玉屏風散中的粗多糖，用Sevag法除蛋白得到精製多糖，另外用乙醇迴流提取藥材粉末後用鏇轉蒸髮儀蒸榦得到醇提濃縮浸膏，然後分彆溶解在去離子水中，用四種不同極性的溶劑萃取，得到四種不同極性部位的濃縮物，將總多糖與四種不同極性溶劑的萃取物分彆用鄰苯三酚自氧化法分彆測定其抗氧化活性。結果：正丁醇層具有較高的超氧陰離子自由基清除率，乙痠乙酯層的清除率次之，玉屏風散水提液的超氧陰離子自由基清除率略高于石油醚層與二氯甲烷層。結論：玉屏風散具有較彊的超氧陰離子自由基清除能力的活性物質的混閤物，其極性可能比較大，易被正丁醇和乙痠乙酯萃取齣來，而被非極性的石油醚和二氯甲烷萃取的量很少。
목적：사선옥병풍산구유항양화활성적유효부위，위약제학이급약이학적연구전정기출。방법：채용수제순침법제취옥병풍산중적조다당，용Sevag법제단백득도정제다당，령외용을순회류제취약재분말후용선전증발의증간득도순제농축침고，연후분별용해재거리자수중，용사충불동겁성적용제췌취，득도사충불동겁성부위적농축물，장총다당여사충불동겁성용제적췌취물분별용린분삼분자양화법분별측정기항양화활성。결과：정정순층구유교고적초양음리자자유기청제솔，을산을지층적청제솔차지，옥병풍산수제액적초양음리자자유기청제솔략고우석유미층여이록갑완층。결론：옥병풍산구유교강적초양음리자자유기청제능력적활성물질적혼합물，기겁성가능비교대，역피정정순화을산을지췌취출래，이피비겁성적석유미화이록갑완췌취적량흔소。
Objective:Screening the antioxidant activeity of Yupingfeng power , which lays the founda-tion for the pharmacy and pharmacology research .Method:The water extraction of Yupingfeng powder in the crude polysaccharide extraction process .According to optimal alcohol precipitation method ,using Sevag meth-od of removing protein was purified polysaccharide , another with ethanol reflux extraction powder by rotary e-vaporator steamed to obtain alcohol extraction of concentrated extract , and then were dissolved in deionized water withfour different the polarity of the solvent extraction , concentrate on four different polar parts , to ex-tract polysaccharide and four kinds of different polar solvents were used to the adjacent benzene three phenol -ic autoxidation method were used to determine the antioxidant activity .Result:The clearance rate of superox-ide anion radical n-butanol layer has higher , ethyl acetate layer clearance times , Yupingfeng powder apron to superoxide anion radical scavenging rate was higher than that of liquid petroleum ether and dichlorometh -ane layer.Conclusion:The mixture of Yupingfeng Powder with superoxide anion free radical scavenging a-bility of the active substance , its polarity may be relatively large , easily by n-butanol and ethyl acetate ex-traction, and seldom be non-polar petroleum ether and dichloromethane extraction amount .
