CAJ | 학술논문

目的：建立测定芍药愈伤组织中两种有效成分--芍药苷和芍药内酯苷--的反相高效液相色谱分析方法。方法：以SinoChrom ODS-BP（250 mm×4.6 mm，5μm）为色谱柱；乙腈-0.05%磷酸二氢钾（v/v，11：89）为流动相，流速为1.0 mL/min，检测波长为230 nm，柱温为30℃。结果：在此色谱条件下芍药苷与芍药内酯苷可完全分离，在一定范围内均表现出良好的线性关系，平均加样回收率（n=5）分别为101.67%和98.59%，RSD均小于2%。结论：本研究建立了一种能快捷简便、准确有效、重现性好的芍药愈伤组织有效成分的检测方法，为进一步优化愈伤组织的培养条件提供了重要保证。
목적：건립측정작약유상조직중량충유효성분--작약감화작약내지감--적반상고효액상색보분석방법。방법：이SinoChrom ODS-BP（250 mm×4.6 mm，5μm）위색보주；을정-0.05%린산이경갑（v/v，11：89）위류동상，류속위1.0 mL/min，검측파장위230 nm，주온위30℃。결과：재차색보조건하작약감여작약내지감가완전분리，재일정범위내균표현출량호적선성관계，평균가양회수솔（n=5）분별위101.67%화98.59%，RSD균소우2%。결론：본연구건립료일충능쾌첩간편、준학유효、중현성호적작약유상조직유효성분적검측방법，위진일보우화유상조직적배양조건제공료중요보증。
AIM:To establish a sensitive RP-HPLC method for determining the active ingredient content of peoniflorin and albiflorin inPaeonia lactifloraPal. calus cultivated in subculture process.METHODS:The separation was performed on SinoChrom ODS-BP column (250 mm×4.6 mm, 5μm), with acetonitrile and 0.05% potassium dihydrogen phosphate solution (v/v, 11:89) as mobile phase, detected at 230 nm wavelength, and the flow rate was 1.0 mL/min.RESULTS:The standard curves of peoniflorin showed a good linearity in a certain range.CONCLUSION:The method is accurate, reliable and suitable for determination of albiflorin and paeoniflorin inPaeoniae radix Alba calus simultaneously. It lays a foundation for further condition optimization of calus culture to produce albiflorin and paeoniflorin inRadix Paeoniae Alba.