CAJ | 학술논문

以罗非鱼链球菌病的病原--海豚链球菌（Streptococcus iniae）为目标菌株，研究了多株分离自罗非鱼养殖水体具有净化水质功能的微生物对其的拮抗作用。结果表明：通过初筛获得了11株对海豚链球菌具有拮抗功能的微生物菌株，其中7株拮抗效果较为显著，分别属于芽孢杆菌(B0903, B0910, B0916)、乳杆菌(L0914, L0917)、假丝酵母(C0909)和诺卡氏菌(N0906)。对这7株菌株从生长优势、共凝集率、净化水质能力等三方面进行进一步的筛选，发现7株菌株的生长系数均大于海豚链球菌，L0917、C0909、N0906的倍增时间显著低于海豚链球菌，且L0914、C0909、N0906能与海豚链球菌产生共凝集作用，共凝集率分别为3.03%、4.11%和1.85%。7株菌都具有一定的净化水质的能力，但C0909、L0914对氨氮的去除率显著低于菌株N0906。研究显示，诺卡氏菌N0906较适宜作为罗非鱼养殖中控制养殖生态环境和链球菌病害的微生态菌株。
이라비어련구균병적병원--해돈련구균（Streptococcus iniae）위목표균주，연구료다주분리자라비어양식수체구유정화수질공능적미생물대기적길항작용。결과표명：통과초사획득료11주대해돈련구균구유길항공능적미생물균주，기중7주길항효과교위현저，분별속우아포간균(B0903, B0910, B0916)、유간균(L0914, L0917)、가사효모(C0909)화낙잡씨균(N0906)。대저7주균주종생장우세、공응집솔、정화수질능력등삼방면진행진일보적사선，발현7주균주적생장계수균대우해돈련구균，L0917、C0909、N0906적배증시간현저저우해돈련구균，차L0914、C0909、N0906능여해돈련구균산생공응집작용，공응집솔분별위3.03%、4.11%화1.85%。7주균도구유일정적정화수질적능력，단C0909、L0914대안담적거제솔현저저우균주N0906。연구현시，낙잡씨균N0906교괄의작위라비어양식중공제양식생태배경화련구균병해적미생태균주。
Eleven strains of antagonistic bacteria against Streptococcus iniae were isolated from the preserved bacteria with the ability of purification of stimulant aquaculture wastewater isolated from the tilapia cultural water. The most compatible strains were chosen for the growth curves, co-agglutination assay and ability of cleanse effects in the aquaculture wastewater. The result showed:7 isolated strains had more advantages in RI than Streptococcus iniae, and shorter doubling time were found in L09017, C0909 and N0906 indicating the growth advantages in the intestine of Streptococcus iniae;3 (L0914, C0909, N0906) of 7 strains had co-agglutination effects with Streptococcus iniae and their co-agglutination ratios were 3.03%, 4.11%and 1.85%, respectively;all 7 strains had the ability of cleanse effects on the aquaculture wastewater, but C0909, L0914 had fewer advantages in degrading ammonia-nitrogen than N0906. In conclusion, N0906 was more moderate to be used as the bio-control bacterium against Streptococcus iniae in tilapia and had good development and research values.