CAJ | 학술논문

应用超高效液相色谱-串联质谱法建立了牛奶中的氯霉素的检测方法。样品经乙酸乙酯提取后，用正已烷除脂，经C18固相萃取小柱富集纯化后，以C18反相柱为分析柱，乙腈-0．5mmol／L乙酸铵溶液为流动相，采用电喷雾负电离源（ESI-），多反应监测模式（SRM），用内标法定量检测。该方法提高了检测灵敏性和分析结果的可靠性，能够适应大规模样品的分析要求。
응용초고효액상색보-천련질보법건립료우내중적록매소적검측방법。양품경을산을지제취후，용정이완제지，경C18고상췌취소주부집순화후，이C18반상주위분석주，을정-0．5mmol／L을산안용액위류동상，채용전분무부전리원（ESI-），다반응감측모식（SRM），용내표법정량검측。해방법제고료검측령민성화분석결과적가고성，능구괄응대규모양품적분석요구。
A method for the detemlination of ehloramphenicol in milk was developed by Ultra Performance Liquid Chromatog- raphy - tanden mass spectrometry( UPLC - MS/MS). The sample were extracted by ethyl acetate and the fat was removed by hexane. The C18 solid phase extraction (SPE) column was use to enrich and purify the chloramphenicol in extraction solu- tion. The chromatographic separation was achieved on a C18 column with a mobile phrase consisting of aeetronitrile and 5mmol/L ethanamide solution. ESI - mode was adopted for the detection of chloramphenicol. And the compound was quan- tified under SRM mode by intemal standard method. The developed approach is characterized by short time consumption for chromatographic separation, so as to meet the requirements for rapid analysis of large - batch samples.