广州化工
廣州化工
엄주화공
GUANGZHOU CHEMICAL INDUSTRY AND TECHNOLOGY
2012年
20期
53-56
,共4页
波叶山蚂蝗%总黄酮%紫外分光光度法%提取工艺
波葉山螞蝗%總黃酮%紫外分光光度法%提取工藝
파협산마황%총황동%자외분광광도법%제취공예
Desmodium sequax Wall%total flavonoids%ultraviolet spectrophotometric method%Extraction process
研究了波叶山蚂蝗（Desmodium sequax Wall）总黄酮的最佳提取工艺。利用单因素实验研究了乙醇回流、超声波两种提取法对波叶山蚂蝗中总黄酮提取率的影响,用紫外分光光度法进行含量测定。结果表明：乙醇回流提取提取法的最佳工艺条件为：提取温度70℃,料液比1∶50 g/mL,乙醇浓度60%,提取时间2.0 h,提取率为2.209%;超声波提取法的最佳工艺条件为：1∶20 g/mL的料液比,70%的乙醇浓度,提取时间40 min,提取率为1.256%。结论乙醇回流提取法的效果较为优越,其提取率远远大于超声波提取法。
研究瞭波葉山螞蝗（Desmodium sequax Wall）總黃酮的最佳提取工藝。利用單因素實驗研究瞭乙醇迴流、超聲波兩種提取法對波葉山螞蝗中總黃酮提取率的影響,用紫外分光光度法進行含量測定。結果錶明：乙醇迴流提取提取法的最佳工藝條件為：提取溫度70℃,料液比1∶50 g/mL,乙醇濃度60%,提取時間2.0 h,提取率為2.209%;超聲波提取法的最佳工藝條件為：1∶20 g/mL的料液比,70%的乙醇濃度,提取時間40 min,提取率為1.256%。結論乙醇迴流提取法的效果較為優越,其提取率遠遠大于超聲波提取法。
연구료파협산마황（Desmodium sequax Wall）총황동적최가제취공예。이용단인소실험연구료을순회류、초성파량충제취법대파협산마황중총황동제취솔적영향,용자외분광광도법진행함량측정。결과표명：을순회류제취제취법적최가공예조건위：제취온도70℃,료액비1∶50 g/mL,을순농도60%,제취시간2.0 h,제취솔위2.209%;초성파제취법적최가공예조건위：1∶20 g/mL적료액비,70%적을순농도,제취시간40 min,제취솔위1.256%。결론을순회류제취법적효과교위우월,기제취솔원원대우초성파제취법。
Optimal extraction parameters of total flavonoids from Desmodium sequax Wall was studied.The effects of two methods including alcohol reflux and Ultrasonic wave on extraction efficiency were investigated by one-factor-at-a-time and the ultraviolet spectrophotometric method was used to determine the content.The results indicated that the optimum conditions of alcohol reflux were as follows: extraction temperature 70 ℃,material/liquid ratio 1:50 g/mL,alcohol concentration 60%,extraction time 2.0 h.The extraction rate was 2.209%,and the optimum conditions of Ultrasonic wave were as below: material/liquid ratio 1:20 g/mL,alcohol concentration 70%,extraction time 40 min.The extraction rate was 1.256%.Alcohol refluxing extraction effect was more superior,and the extraction yield was far greater than Ultrasonic wave.
