现代农业科技
現代農業科技
현대농업과기
XIANDAIHUA NONGYE
2012年
20期
84-85
,共2页
苏永昌%阮伟达%刘淑集%王茵%吴成业
囌永昌%阮偉達%劉淑集%王茵%吳成業
소영창%원위체%류숙집%왕인%오성업
海参%总皂苷%测定方法
海參%總皂苷%測定方法
해삼%총조감%측정방법
sea cucumber%total saponin%determination
以人参皂苷Re为标准对照,香草醛—冰醋酸—高氯酸作为显色体系,建立地瓜参皂苷含量测定方法,结果表明:皂苷浓度在40~200μg/mL时,与吸光度值呈良好线性关系,加标回收率为101.9%,RSD=4.63%,精密度试验RSD=1.77%,稳定性试验RSD=3.16%,重复性试验RSD=3.15%,说明方法学各指标均符合要求。
以人參皂苷Re為標準對照,香草醛—冰醋痠—高氯痠作為顯色體繫,建立地瓜參皂苷含量測定方法,結果錶明:皂苷濃度在40~200μg/mL時,與吸光度值呈良好線性關繫,加標迴收率為101.9%,RSD=4.63%,精密度試驗RSD=1.77%,穩定性試驗RSD=3.16%,重複性試驗RSD=3.15%,說明方法學各指標均符閤要求。
이인삼조감Re위표준대조,향초철—빙작산—고록산작위현색체계,건입지과삼조감함량측정방법,결과표명:조감농도재40~200μg/mL시,여흡광도치정량호선성관계,가표회수솔위101.9%,RSD=4.63%,정밀도시험RSD=1.77%,은정성시험RSD=3.16%,중복성시험RSD=3.15%,설명방법학각지표균부합요구。
A quantitative analysis of the Acaudina molpadioides method was established by using the ginseng saponins Re as standard and vanillin—glacial acetic acid—high chlorine acid as color-forming reagents.The results showed that concentration had a good relationship with the absorbance when it was among 40-200 μg/mL.The average recovery was 101.9%,RSD=4.63%.Precision experiments RSD=1.77%,stability experiments RSD=3.16%,repetitive experiments RSD=3.15%,explained that the indexes of methodology met the requirements.