CAJ | 학술논문

目的：观察利用胰岛素严格控制血糖对糖尿病性勃起功能障碍（DMED）防治效果。方法年龄8周的健康Sprague-Dawley（SD）大鼠，STZ腹腔注射诱导糖尿病，监测血糖两个月诱导DMED模型，分正常对照组（NC），糖尿病安慰剂治疗组（DM），糖尿病胰岛素治疗组（DM-Inshulin）胰岛素每次2~6个单位，一天2次腹腔注射，连续治疗4周，早晚监测血糖。治疗结束后检测阴茎海绵体神经刺激下阴茎海绵体内压与平均动脉血压（ICP/MAP）评估勃起功能。留取阴茎组织进行组织学检测。结果 DM-Insulin组大鼠血糖和糖化血红蛋白得到有效控制，虽然勃起功能（以ICP/MAP检测）比较DM 组有所改善，但是比较NC组显著降低。DM-Insulin组大鼠阴茎海绵体内皮细胞标记物vWF、平滑肌等组织学病理变化及组织细胞凋亡指数比较DM组显著改善，但仍未恢复至NC组水平。结论糖尿病大鼠利用胰岛素严格控制血糖水平，可部分改善阴茎勃起功能和阴茎海绵体病理变化，但是不能完全修复勃起功能障碍的病理变化，这可能与糖尿病糖基化终末产物及其受体（AGE-RAGE）相关代谢记忆有关，有待于进一步深入研究。
목적：관찰이용이도소엄격공제혈당대당뇨병성발기공능장애（DMED）방치효과。방법년령8주적건강Sprague-Dawley（SD）대서，STZ복강주사유도당뇨병，감측혈당량개월유도DMED모형，분정상대조조（NC），당뇨병안위제치료조（DM），당뇨병이도소치료조（DM-Inshulin）이도소매차2~6개단위，일천2차복강주사，련속치료4주，조만감측혈당。치료결속후검측음경해면체신경자격하음경해면체내압여평균동맥혈압（ICP/MAP）평고발기공능。류취음경조직진행조직학검측。결과 DM-Insulin조대서혈당화당화혈홍단백득도유효공제，수연발기공능（이ICP/MAP검측）비교DM 조유소개선，단시비교NC조현저강저。DM-Insulin조대서음경해면체내피세포표기물vWF、평활기등조직학병리변화급조직세포조망지수비교DM조현저개선，단잉미회복지NC조수평。결론당뇨병대서이용이도소엄격공제혈당수평，가부분개선음경발기공능화음경해면체병리변화，단시불능완전수복발기공능장애적병리변화，저가능여당뇨병당기화종말산물급기수체（AGE-RAGE）상관대사기억유관，유대우진일보심입연구。
Objective To investigate the therapeutic effects of insulin on eretile dysfunction in a rat model with streptozotocin-induced diabetes. Methods The diabetic erectile dysfunction (DMED) rat model was made by injecting the male 8-week-old Sprague-Dawley rats intraperitoneally with vehicle or freshly prepared 60 mg/kg streptozocin, and blood glucose level was measured in the later experiments. Then the rats were divided into three groups: the normal control group (N), the diabetes group (DM) and the diabetes plus insulin therapy group (DM+Insulin). 8 weeks after STZ injection, the DM+Insulin group were treated with 2~6 units of neutral protamine Hagedorn twice a day for 4 weeks through subcutaneous injection. After the final treatment, all rats were tested for erectile function by measuring the intracavernous pressure and mean arterial pressure (ICP/MAP), and the penile was harvested for histology study. Results Although the glycemic level was tightly controlled by insulin in the therapy group, ICP/MAP level was partially restored compared to that of the normal control group, so with the endothelial and smooth muscle contents and apoptosis index. Conclusion Insulin could partially restore the erectile functions and pathology changes in the streptozotocin-induced diabetes rat model. Further studies are needed to investigate the underlying mechanisms in the processes of diabetic erectile dysfunction.