CAJ | 학술논문

PRRSV易引起基因变异现象,使得异地疫苗不能有效地控制本地区PRRS的发生。为研制适合本地区的亚单位疫苗,试验参照GeneBank中已发表的PRRSV序列设计了1对特异性引物,对吉林分离株JL-0605对GP5基因进行了序列扩增和测序,序列分析结果表明,PRRSV JL-0605株GP5氨基酸序列与CH-1α和HEB1株氨基酸序列高度一致,而与VR-2332和BJ-4株有一定的差异,和LV株在N端及中心区具有很大的差异,JL-0605株GP5蛋白抗原性及亲水性与中国河北株具有较高的相似性;与VR-2332株相比,30～40位氨基酸处抗原表位形态发生较大变化,在135～145位氨基酸处抗原表位优势增强现象。
PRRSV역인기기인변이현상,사득이지역묘불능유효지공제본지구PRRS적발생。위연제괄합본지구적아단위역묘,시험삼조GeneBank중이발표적PRRSV서렬설계료1대특이성인물,대길림분리주JL-0605대GP5기인진행료서렬확증화측서,서렬분석결과표명,PRRSV JL-0605주GP5안기산서렬여CH-1α화HEB1주안기산서렬고도일치,이여VR-2332화BJ-4주유일정적차이,화LV주재N단급중심구구유흔대적차이,JL-0605주GP5단백항원성급친수성여중국하북주구유교고적상사성;여VR-2332주상비,30～40위안기산처항원표위형태발생교대변화,재135～145위안기산처항원표위우세증강현상。
PRRSV can lead to gene variation easily,and different vaccine can not stop the outbreak of PRRS in native place effectively.In order to study and find appropriate subunit vaccine for native place,experiment used specific primers which were designed according to the PRRSV sequence published by Genebank,and amplified and sequenced the DNA of major structural proteins GP5 of isolate plant JL-0605 existing in Jilin Province.The results showed that GP5 amino acid sequence of plant PRRSV JL-0605 has the same height with plant CH-1α and plant HEB1,but a little different with plant VR-2332 and plant BJ-4,especially with plant LV in the protein N and middle.The antigenicity and hydrophile of protein GP5,plant JL-0605 are much similar with the plant from Hebei Province,China.But compared with plant VR-2332,its Epitope morphology in amino acids sequence 30 to 40 has changed a lot,and enlarged its Epitope dominance in amino acids sequence 135 to 145.