CAJ | 학술논문

目的研究刺五加叶不同部位在不同浓度下的体外抗氧化活性。方法采用清除ABTS＋自由基、DPPH自由基评价法和测定还原性法,利用紫外分光光度计法测定刺五加叶的总提液、10%、30%和50%大孔吸附树脂醇洗脱部位在不同浓度下的抗氧化能力。结果刺五加叶不同部位抗氧化活性的强弱顺序为：30%部位〉10%部位〉总提液〉50%部位,随着各部位浓度的增加,抗氧化能力增强。ABTS＋自由基和DPPH自由基模型中,在浓度为0.64mg/mL时,30%的部位对ABTS＋DPPH自由基模最大清除率分别达到99.541%、95.331%,与同浓度VC的清除率接近,其次浓度0.64mg/mL的10%部位的最大清除率分别达到96.324%、88.033%。在还原性法测定中30%部位在浓度为0.64mg/mL时,吸光度为2.269,与VC较接近,同浓度时10%部位的吸光度为2.155,次于30%部位。结论刺五加叶抗氧化能力较好,各种评定方法结合分析,其30%部位最好,可以开发为抗氧化剂,为进一步研究和开发刺五加叶提供了科学依据。
목적연구자오가협불동부위재불동농도하적체외항양화활성。방법채용청제ABTS＋자유기、DPPH자유기평개법화측정환원성법,이용자외분광광도계법측정자오가협적총제액、10%、30%화50%대공흡부수지순세탈부위재불동농도하적항양화능력。결과자오가협불동부위항양화활성적강약순서위：30%부위〉10%부위〉총제액〉50%부위,수착각부위농도적증가,항양화능력증강。ABTS＋자유기화DPPH자유기모형중,재농도위0.64mg/mL시,30%적부위대ABTS＋DPPH자유기모최대청제솔분별체도99.541%、95.331%,여동농도VC적청제솔접근,기차농도0.64mg/mL적10%부위적최대청제솔분별체도96.324%、88.033%。재환원성법측정중30%부위재농도위0.64mg/mL시,흡광도위2.269,여VC교접근,동농도시10%부위적흡광도위2.155,차우30%부위。결론자오가협항양화능력교호,각충평정방법결합분석,기30%부위최호,가이개발위항양화제,위진일보연구화개발자오가협제공료과학의거。
Objective Study on different Fractions of Acanthopanax Senticosus(Rupr.etMaxim.) Harms Leaves at different concentrations,and determine its antioxidative activities.Methods The antioxidative activities of Acanthopanax Senticosus compared with VC were investigated employing various methods established in vitro systems such as DPPH(1,1-diphenyl-2-picrylhydrazyl) radical ABTS(2,2-azo-bis-(3-ethylbenzothiozoline-6-sulphonic acid) radical scavenging assay and reducing power assay measured by ultraviolet spectro-photometer.Results The elution fractions of Acanthopanax Senticosus exhibited significant antioxidant properties on ABTS free radical,DPPH radical scavenging systems,and good reduction.The inhibition of freeing radical scavenging ability of the different elution fractions from strong to weak is: 30%fraction10%fraction the total extracts50%fraction.The antioxidant properties of different fractions depended well on the their dose.With the increasing of the concentration various fractions,the antioxidative capacity was enhanced.The inhibition of ABTS radical scavenging was 99.541% at the concentration of 0.64mg/mL in 30% fraction,and the inhibition of DPPH radical scavenging was 95.331% at the same concentration in 30% fraction,both close to VC.The inhibition in 10% fraction was weaker than 30% fraction,it was 96.324%、88.033% respectively.In the reducing power assay,the absorbance was about closed to VC that was 2.269 of 30% fraction,and the 10% fraction was 2.155.Conclusion Different fractions of Acanthopanax Senticosus showed significant antioxidant properties,the 30% ethanol fraction exhibited highest antioxidant activities in different investigation systems.The present studies offered a scientific record that 30% ethanol fraction would be developped to be an antioxidant.