CAJ | 학술논문

目的应用组织培养技术对平贝母进行离体培养,从而得到愈伤组织和不定芽。方法以鳞茎为材料诱导愈伤组织与不定芽,采用正交实验设计确定最佳的激素种类与浓度。结果适当的激素组合及配比可诱导出愈伤组织和不定芽,最高的诱导率可分别达到14%以上、86.7%。结论愈伤组织最佳诱导培养基为MS＋2,4-D 1.0mg/L＋6-BA 0.9mg/L＋NAA 0.5mg/L;最佳继代培养基为MS＋2,4-D0.5mg/L＋6-BA 0.3mg/L;不定芽最佳诱导培养基为MS＋KT 0.8mg/L＋NAA 0.3mg/L。
목적응용조직배양기술대평패모진행리체배양,종이득도유상조직화불정아。방법이린경위재료유도유상조직여불정아,채용정교실험설계학정최가적격소충류여농도。결과괄당적격소조합급배비가유도출유상조직화불정아,최고적유도솔가분별체도14%이상、86.7%。결론유상조직최가유도배양기위MS＋2,4-D 1.0mg/L＋6-BA 0.9mg/L＋NAA 0.5mg/L;최가계대배양기위MS＋2,4-D0.5mg/L＋6-BA 0.3mg/L;불정아최가유도배양기위MS＋KT 0.8mg/L＋NAA 0.3mg/L。
Objective To apply the tissue culture technology for the callus and adventitious buds of Fritillaria ussurionsis Maxim in vitro.Methods The optimum hormone types and concentration for the callus and adventitious bud from the bulbs were determined with the orthogonal experiment.Results The optimum hormone types and concentration could induce callus and adventitious bud,the highest inducing rates are more than 14%,86.7% respectively.Conclusion The optimum callus induced media: MS+2,4-D 1.0mg/L+6-BA 0.9mg/L+NAA 0.5mg/L,the successive transfer media: MS+2,4-D 0.5mg/L+6-BA 0.3mg/L;the adventitious bud media: MS+KT 0.8mg/L+NAA 0.3mg/L.