青海师范大学学报：自然科学版
青海師範大學學報：自然科學版
청해사범대학학보：자연과학판
Journal of Qinghai Normal University(Natural Science Edition)
2012年
3期
65-67
,共3页
陈晓红%陈元涛%钱蔚%宋青云
陳曉紅%陳元濤%錢蔚%宋青雲
진효홍%진원도%전위%송청운
HPLC%长毛风毛菊%木犀草素%异鼠李素%同时%测定
HPLC%長毛風毛菊%木犀草素%異鼠李素%同時%測定
HPLC%장모풍모국%목서초소%이서리소%동시%측정
HPLC%saussurea hieracioides hook%lsorhamnetin%luteolin%simultaneous%determination
目的采用HPLC同时测定长毛风毛菊中木犀草素和异鼠李素的含量.方法 Eclipse XOB-C18（150×4.6mm,5μm）色谱柱,甲醇和水（含0.05%的磷酸）梯度洗脱,流速1mL.min-1,检测波长350nm,柱温35℃.结果木犀草素和异鼠李素的线性范围分别为0.106～5.81μg（r=0.9996）,0.113～6.94μg（r=0.9997）;平均回收率分别为99.14%（RSD=1.47%）和98.96%（RSD=0.99%）.结论该方法可用于藏药长毛风毛菊中木犀草素和异鼠李素含量的同时快速、准确测定.
目的採用HPLC同時測定長毛風毛菊中木犀草素和異鼠李素的含量.方法 Eclipse XOB-C18（150×4.6mm,5μm）色譜柱,甲醇和水（含0.05%的燐痠）梯度洗脫,流速1mL.min-1,檢測波長350nm,柱溫35℃.結果木犀草素和異鼠李素的線性範圍分彆為0.106～5.81μg（r=0.9996）,0.113～6.94μg（r=0.9997）;平均迴收率分彆為99.14%（RSD=1.47%）和98.96%（RSD=0.99%）.結論該方法可用于藏藥長毛風毛菊中木犀草素和異鼠李素含量的同時快速、準確測定.
목적채용HPLC동시측정장모풍모국중목서초소화이서리소적함량.방법 Eclipse XOB-C18（150×4.6mm,5μm）색보주,갑순화수（함0.05%적린산）제도세탈,류속1mL.min-1,검측파장350nm,주온35℃.결과목서초소화이서리소적선성범위분별위0.106～5.81μg（r=0.9996）,0.113～6.94μg（r=0.9997）;평균회수솔분별위99.14%（RSD=1.47%）화98.96%（RSD=0.99%）.결론해방법가용우장약장모풍모국중목서초소화이서리소함량적동시쾌속、준학측정.
Objective To establish a quantitative method of simultaneously determination of luteolin and lsorhamnetin in Saussurea hieracioides Hook.f by HPLC.Methods The sample was separated on column of Eclipse XOB-C18(150×4.6 mm,5μm)with gradient elution,the mobile phase was comprised of methanol and water(0.05% phosphoric acid).The flow rate was 1mL·min-1.The detection wavelength was at 350 nm and the column temperature was 35 ℃.Results The linear ranges of luteolin and lsorhamnetin were 0.106~5.81 μg(r=0.9996),0.113~6.94μg(r=0.9997)respectively;The average recoveries were 99.14%(RSD=1.47%)and 98.96%(RSD=0.99%),respectively.Conclusions The method is rapid and precise.
