CAJ | 학술논문

采用3种消毒时间对沙冬青、老瓜头、甘草3种沙生药用植物内生真菌进行分离．结果表明：消毒3rain来消除非内生菌的影响，效果较好；同一植株分离出内生真菌的数量，以根部最多，其次是茎部，叶部最少．合并形态相同的分离物，分离出的124株内生真菌除24株无孢茵属外，经初步鉴定分属于13个属，卵形孢霉属（Oospora）和串珠霉属（Monilia）各占18．63％；交链孢霉（Alternaria）占12．96％；间毛孢霉属（Septosporium）占lO．53％；镰孢霉属（Fusarium）占9．72％；而头孢霉属（Cephalosporium）、柬丝菌属（Ozonium）、青霉属（Penicillium）、曲霉属（Aspergillus）、地霉属（Geotrichum）、亚大茎点菌属（Macrophoming）、丝核菌属（Rhizoctonia）分离数均小于5％．以4种植物病原菌为靶标菌，筛选出5株高活性拮抗菌株，抑菌带宽度达14．99mm．
채용3충소독시간대사동청、로과두、감초3충사생약용식물내생진균진행분리．결과표명：소독3rain래소제비내생균적영향，효과교호；동일식주분리출내생진균적수량，이근부최다，기차시경부，협부최소．합병형태상동적분리물，분리출적124주내생진균제24주무포인속외，경초보감정분속우13개속，란형포매속（Oospora）화천주매속（Monilia）각점18．63％；교련포매（Alternaria）점12．96％；간모포매속（Septosporium）점lO．53％；렴포매속（Fusarium）점9．72％；이두포매속（Cephalosporium）、간사균속（Ozonium）、청매속（Penicillium）、곡매속（Aspergillus）、지매속（Geotrichum）、아대경점균속（Macrophoming）、사핵균속（Rhizoctonia）분리수균소우5％．이4충식물병원균위파표균，사선출5주고활성길항균주，억균대관도체14．99mm．
Using 3 different disinfect time to isolate the endophytic fungi in three psammonphilous medical plant. Ammopiptanthus rnongolicus , Glycyrriza uralensia and Cynanchum licorice. The results indicated that the disinfection effect of endophytic bacteria in 3 min to eliminate non-works better; quantities of endophytic fungi isolated from the same plant, roots took the largest number, followed by the stem, leaves at least. Combining the same isolates from the seeds, roots, stems and leaves, 124 endophytic actinomycetes were identified belonging to 13 genera except 24 of them, Oospora and Monilia, each of them accounts for 18. 63%; Alternaria accounts for 12. 96%; Septosporium accounts for 10. 53%; Fusarium accounts for 9.72%; the isolate rate of Cephalosporium, Ozonium ,Penicillium, Aspergillus, Geotrichum, Macrophoming and Rhizoctonia is less than 5%. Using 4 kinds of pathogenic bacteria as target bacteria, screened out 5 antagonistic strains with high activity, antibacterial bandwidth up to 14.99 mm.