花生学报
花生學報
화생학보
PEANUT SCIENCE AND TECHNOLOGY
2012年
3期
21-26
,共6页
杨传得%关淑艳%唐月异%王秀贞%吴琪%宫清轩%王传堂
楊傳得%關淑豔%唐月異%王秀貞%吳琪%宮清軒%王傳堂
양전득%관숙염%당월이%왕수정%오기%궁청헌%왕전당
花生%GC%脂肪酸%测定
花生%GC%脂肪痠%測定
화생%GC%지방산%측정
groundnut%GC%fatty acid%determination
描述了一种利用5～20mg花生种子组织测定花生8种脂肪酸的方法。于室温下制备用于色谱分析的样品耗时35min,气相色谱分析则需要18min。研究结果证实,本法准确性高,重复性较好,可满足花生遗传育种研究中对种子无损取样及基因聚合育种需要,将为花生脂肪酸成分的遗传改良提供便利。
描述瞭一種利用5～20mg花生種子組織測定花生8種脂肪痠的方法。于室溫下製備用于色譜分析的樣品耗時35min,氣相色譜分析則需要18min。研究結果證實,本法準確性高,重複性較好,可滿足花生遺傳育種研究中對種子無損取樣及基因聚閤育種需要,將為花生脂肪痠成分的遺傳改良提供便利。
묘술료일충이용5～20mg화생충자조직측정화생8충지방산적방법。우실온하제비용우색보분석적양품모시35min,기상색보분석칙수요18min。연구결과증실,본법준학성고,중복성교호,가만족화생유전육충연구중대충자무손취양급기인취합육충수요,장위화생지방산성분적유전개량제공편리。
A protocol for analyzing 8 fatty acids using 5-20 mg groundnut seed tissue was described, which required 35 min for preparation at room temperature and 18 min for gas chromatography analy- sis. The results of analysis proved to be accurate and reproducible, irrespective of sampling amount and fatty acid content. The protocol caters the need for non-destructive sampling and gene pyramiding in genetic and breeding studies in groundnut, and may facilitate genetic improvement in fatty acid composition of the groundnut crop.
