CAJ | 학술논문

采用琼脂稀释法观察了10种常见中草药水提物对强致病性嗜水气单胞菌ZHYYZ-1的体外抑菌效果，结果表明：（1）五倍子、黄芩、金银花、蒲公英的抑菌效果明显优于连翘、黄连、穿心莲、鱼腥草、板蓝根和大青叶；（2）菌浓度1．0×10^4CFU／mL条件下，五倍子、黄芩、金银花、蒲公英的最小抑菌浓度依次为0．20mg／mL、1．56mg／mL、1．56mg／mL和3．12mg／mL；（3）中草药两两组配，表现抑菌协同效应的组合及条件为：茵浓度1．0×10^7CFU／mL时，蒲公英-黄芩组合，黄芩-金银花组合及蒲公英与五倍子配比为3：2或黄芩与五倍子配比大于等于3：2时的组合；菌浓度1．0）〈106CFU／mL时，蒲公英-黄芩组合，黄芩-金银花组合及蒲公英与金银花的配比小于等于3：2或黄芩与五倍子的配比大于等于3：2时的组合；菌浓度为1．0×10^5CFU／mL时，黄芩一金银花组合及蒲公英与金银花的配比小于等于3：2时的组合。
채용경지희석법관찰료10충상견중초약수제물대강치병성기수기단포균ZHYYZ-1적체외억균효과，결과표명：（1）오배자、황금、금은화、포공영적억균효과명현우우련교、황련、천심련、어성초、판람근화대청협；（2）균농도1．0×10^4CFU／mL조건하，오배자、황금、금은화、포공영적최소억균농도의차위0．20mg／mL、1．56mg／mL、1．56mg／mL화3．12mg／mL；（3）중초약량량조배，표현억균협동효응적조합급조건위：인농도1．0×10^7CFU／mL시，포공영-황금조합，황금-금은화조합급포공영여오배자배비위3：2혹황금여오배자배비대우등우3：2시적조합；균농도1．0）〈106CFU／mL시，포공영-황금조합，황금-금은화조합급포공영여금은화적배비소우등우3：2혹황금여오배자적배비대우등우3：2시적조합；균농도위1．0×10^5CFU／mL시，황금일금은화조합급포공영여금은화적배비소우등우3：2시적조합。
Using agar dilution method, antibacterial effect in vitro of ten common Chinese herbs on highly pathogenic Aeromonas hydrophila ZHYYZ-1 were observed. The results were that:(1) the antibacterial effects of Chinese Gall, Baical Skullcap Root, Honeysuckle Flower and Mongolian Dandelion Herb were demonstrably superior to Forsythia suspensa, Coptis Root, Common Andrographis Herb, Heartleaf Houttuymia Herb, Indigowoad Root and Indigowoad Leaf.(2) when the concentration of A. hydrophila ZHYYZ-1 was 1.0×10^4 CFU/mL, the minimal inhibitory concentration (MIC) of Chinese Gall, Baical Skullcap Root, Honeysuckle Flower and Mongolian Dandelion Herb were 0.20 mg/mL, 1.56 mg/mL, 1.56 mg/mL and 3.12 mg/mL, respectively; (3) the combined conditions of two Chinese herbs displaying the antibacterial synergistic effects were that: the compositions ratio of Mongolian Dandelion Herb and Baical Skullcap Root, Baical Skullcap Root and Honeysuckle Flower, Mongolian Dandelion Herb and Chinese Gall were separately 3:2, or the proportion of Baical Skullcap Root and Chinese Gall was more than 3:2, as the concentration of A. hydrophila ZHYYZ-1 was 1.0×10^7 CFU/mL; the compositions ratio of Mongolian Dandelion Herb and Baical Skullcap Root, Baical Skullcap Root and Honeysuckle Flower, Mongolian Dandelion Herb and Honeysuckle Flower were separately less than 3:2, or the proportion of Baical Skullcap Root and Chinese Gall was more than 3:2, as the concentration of A. hydrophila ZHYYZ-1 was 1.0×10^6 CFU/mL; the composition ratio of Baical Skullcap Root and Honeysuckle Flower, or the proportion of Mongolian Dandelion Herb and Honeysuckle Flower were less than 3:2 respectively in the condition that the concentration of A. hydrophila ZHYYZ-1 was 1.O×10^5 CFU/mL.