CAJ | 학술논문

根据GeneBank上发表的单核细胞增多性李氏杆菌（Listeriamonocytogenes，LM）的内化素B（intemalinB，inlB）和肌动蛋白A（actinA，actA）基因设计特异性引物，对5株不同来源的健康绵羊单核细胞增多性李氏杆菌和一株临床分离的单核细胞增多性李氏杆菌的inlB及actA基因进行PCR扩增，克隆测序分析其序列，并对2株部分基因缺失的单增李氏杆菌进行小鼠攻毒试验。结果表明：5株健康绵羊分离株单增李氏杆菌与临床分离株有较高的同源性，并且发现2株部分基因缺失的单增李氏杆菌；小鼠攻毒试验表明缺失株毒力有降低，但是不明显。
근거GeneBank상발표적단핵세포증다성리씨간균（Listeriamonocytogenes，LM）적내화소B（intemalinB，inlB）화기동단백A（actinA，actA）기인설계특이성인물，대5주불동래원적건강면양단핵세포증다성리씨간균화일주림상분리적단핵세포증다성리씨간균적inlB급actA기인진행PCR확증，극륭측서분석기서렬，병대2주부분기인결실적단증리씨간균진행소서공독시험。결과표명：5주건강면양분리주단증리씨간균여림상분리주유교고적동원성，병차발현2주부분기인결실적단증리씨간균；소서공독시험표명결실주독력유강저，단시불명현。
The specific primers were designed based on internalin B (inlB) and actin A (actA) genes of L isteria monocytogenes published in the GeneBank. Then inlB and actA genes were amplified from 5 Listeria monocytogenes strains isolated from clinically healthy sheep and one strain from diseased sheep. In addition, two Listeria monocytogenes strains with partial gene deletion were inoculated into mice to examine their virulence. The result showed that 5 strains from healthy sheep shared high homology with that strain from clinical case. The virulence of partial gene deletion strains were reduced but not significant.