CAJ | 학술논문

　　为简便有效地鉴定党参药材及其混伪品并验证ITS/ITS2序列作为DNA条形码鉴定药材的稳定性和准确性，本研究选用党参药材及其混伪品作为研究对象，对33份药材样本提取基因组DNA，通过PCR扩增ITS序列，采用比对法、最小距离法对序列鉴定能力进行评估。通过序列比对，分析变异位点信息确定不同的单倍型并计算种内和种间K2P距离。ITS2序列采用基于隐马尔可夫模型的HMMer（Hidden Markov Model）注释方法获得。结果表明，党参药材3个基原物种ITS序列长度为654-655 bp，ITS2序列长度均为239 bp，ITS/ITS2序列种内平均K2P遗传距离均远小于其与混伪品的种间平均K2P遗传距离，因此ITS/ITS2序列作为DNA条形码能稳定、准确鉴别党参药材及其混伪品，为其鉴定提供新的技术手段。
　　위간편유효지감정당삼약재급기혼위품병험증ITS/ITS2서렬작위DNA조형마감정약재적은정성화준학성，본연구선용당삼약재급기혼위품작위연구대상，대33빈약재양본제취기인조DNA，통과PCR확증ITS서렬，채용비대법、최소거리법대서렬감정능력진행평고。통과서렬비대，분석변이위점신식학정불동적단배형병계산충내화충간K2P거리。ITS2서렬채용기우은마이가부모형적HMMer（Hidden Markov Model）주석방법획득。결과표명，당삼약재3개기원물충ITS서렬장도위654-655 bp，ITS2서렬장도균위239 bp，ITS/ITS2서렬충내평균K2P유전거리균원소우기여혼위품적충간평균K2P유전거리，인차ITS/ITS2서렬작위DNA조형마능은정、준학감별당삼약재급기혼위품，위기감정제공신적기술수단。
The ITS/ITS2 barcodes were used to simply and effectively identify Codonopsis Radix and its adulter-ants. In this study, ITS (internal transcribed spacer of unclear ribosomal DNA) regions were amplified using PCR (polymerase chain reaction) from thirty-three samples of Codonopsis Radix and ITS2 regions were obtained from the ITS sequences using the hidden Markov model (HMMer)-based annotation methods. The sequences of ITS/ITS2 regions were aligned and the genetic distances were computed by MEGA5.0. Species identification efficiency of ITS/ITS2 sequences were evaluated using BLAST1 and nearest distance methods. The results indicated that The sequences lengths of ITS regions of Codonopsis Radix were 654-655 bp, and the lengths of ITS2 regions were 239 bp. The intraspecific genetic distances among Codonopsis Radix were obviously lower than the interspecific genetic distance between Codonopsis Radix and its adulterants. Therefore, ITS/ITS2 regions can stably and accu-rately distinguish Codonopsis Radix and its adulterants.