CAJ | 학술논문

　　目的：对中药材佩兰及其混伪品进行ITS2条形码鉴定，以确保该药材的质量以及临床疗效。方法：提取佩兰的DNA，利用PCR技术扩增其ITS2序列，双向测序，所得序列经过CodonCode Aligner拼接后，用软件MEGA5进行相关数据分析，计算其种内、种间遗传距离，并构建系统发育树。结果：佩兰药材的ITS2序列长度为218 bp；佩兰种内最大K2P距离为0.009，与混伪品种间最小K2P距离为0.024。ITS2序列的NJ系统聚类树可明显区分佩兰药材及其混伪品，表现出良好的单系性。结论：ITS2条形码序列能够成功鉴定中药材佩兰与其混伪品，为保障临床安全用药提供了新的技术方法。
　　목적：대중약재패란급기혼위품진행ITS2조형마감정，이학보해약재적질량이급림상료효。방법：제취패란적DNA，이용PCR기술확증기ITS2서렬，쌍향측서，소득서렬경과CodonCode Aligner병접후，용연건MEGA5진행상관수거분석，계산기충내、충간유전거리，병구건계통발육수。결과：패란약재적ITS2서렬장도위218 bp；패란충내최대K2P거리위0.009，여혼위품충간최소K2P거리위0.024。ITS2서렬적NJ계통취류수가명현구분패란약재급기혼위품，표현출량호적단계성。결론：ITS2조형마서렬능구성공감정중약재패란여기혼위품，위보장림상안전용약제공료신적기술방법。
Objective: This study aimed to discriminate between Eupatorii Herba and its adulterants in order to guarantee the quality and clinical curative effect of this medicinal material. Methods: Genomic DNA extracted from Eupatorii Herba was used as templates. The internal transcribed spacer 2 (ITS2) of nuclear ribosomal DNA was amplified. Sequence assembly and consensus sequence generation were performed by CodonCode Aligner. The intraspecific and interspecific genetic distances of Eupatorii Herba and its adulterants were computed by MEGA5 and the phylogenetic tree was constructed using the neighbor-joining (NJ) method. Results: The length of ITS2 sequence of Eupatorii Herba was 218 bp. The maximum intraspecific genetic distance (K2P distance) of Eupatorii Herba was 0.0092. The minimum interspecific genetic distance of Eupatorii Herba and its adulterants was 0.024. The NJ trees showed that the ITS2 sequence would be used to identify Eupatorii Herba and its adulterants. Con-clusion: ITS2 sequence was able to identify Eupatorii Herba and its adulterants correctly and it provided a new technique to ensure clinical safety in utilization of traditional Chinese medicines.