CAJ | 학술논문

　　为验证DNA条形码序列在物种鉴定方面及物种的系统发育方面的广适性，本研究以黎药植物木麻黄为研究对象，收集木麻黄及其近缘种和混伪品22份样本，采用4对引物（ITS，ITS2， trnH-psbA ，rbcL）分别进行PCR扩增，产物进行双向测序。利用CodonCode Aligner 软件进行序列的拼接，采用MEGA5.1进行数据的处理。结果表明，利用ITS/ITS2可以区分木麻黄与其近缘种以及混伪品，样品的psbA-trnH，rbcL 和ITS/ITS24个序列中，ITS/ITS2序列具有明显的 DNA gap。基于 ITS/ITS2序列构建的NJ树结果表明，木麻黄属所有样品聚为一类，呈现明显的单系性，与其亲缘关系较近的是藤黄科。因此，ITS/ITS2序列可以准确鉴定黎药的基原植物。并能从分子水平揭示木麻黄的系统演化规律。
　　위험증DNA조형마서렬재물충감정방면급물충적계통발육방면적엄괄성，본연구이려약식물목마황위연구대상，수집목마황급기근연충화혼위품22빈양본，채용4대인물（ITS，ITS2， trnH-psbA ，rbcL）분별진행PCR확증，산물진행쌍향측서。이용CodonCode Aligner 연건진행서렬적병접，채용MEGA5.1진행수거적처리。결과표명，이용ITS/ITS2가이구분목마황여기근연충이급혼위품，양품적psbA-trnH，rbcL 화ITS/ITS24개서렬중，ITS/ITS2서렬구유명현적 DNA gap。기우 ITS/ITS2서렬구건적NJ수결과표명，목마황속소유양품취위일류，정현명현적단계성，여기친연관계교근적시등황과。인차，ITS/ITS2서렬가이준학감정려약적기원식물。병능종분자수평게시목마황적계통연화규률。
In this research, Casuarina eguisetifolia Linn was used to verify the broadly suitability of DNA bar-codes in identification of Li-medicine plants and systematic development of species. The genomic DNA of 22 samples collected C. eguisetifolia and its adulterants were amplified by 4 pairs of primers respectively (ITS (inter-nal transcribed spacer), ITS2 (internal transcribed spacer 2), trnH-psbA , rbcL) and sequenced bi-directionally. Obtained sequences were assembled using CodonCode Aligner. The dates were analysised using MEGA5.1 in ac-cordance with the kimura 2-parameter (K2P) model. The neighbor-joining (NJ) phylogenetic trees were construct-ed. Our study demonstrated the efficacy of ITS/ITS2 to distinguish between C. eguisetifolia and other adulterants species at the molecular level. Comparative to the primer of trnH-psbA and rbcL, there was a obviously DNA gap. The NJ trees showed that the several species of Casuarina can be classified to same types to show a obvi-ously monophyly, which the nearest family was Guttiferae. Therefore, ITS/ITS2 regions can accurately distinguish the original plant of Li-medicine. The systematic evolution of Casuarina can be verified in the molecular level.