CAJ | 학술논문

　　目的：本文选用土荆皮及其5种伪品共37份材料，对ITS2序列进行比较研究。方法：采用CodonCode Aligner3.5.7进行序列拼接，MEGA5.0计算土荆皮的种内及其与混伪品的种间 K2P距离，构建NJ分子系统树，并分析土荆皮及其混伪品ITS2序列二级结构的分子形态学特征。结果：土荆皮药材ITS2序列单倍型与其基原植物叶片序列一致；土荆皮及其混伪品的ITS2序列种间遗传距离远大于土荆皮种内样本的遗传距离；NJ树显示土荆皮样品单独聚为一支，药材土荆皮正伪品的ITS2序列二级结构差异显著。结论：ITS2可以作为土荆皮及其伪品鉴定的有效DNA条形码序列。
　　목적：본문선용토형피급기5충위품공37빈재료，대ITS2서렬진행비교연구。방법：채용CodonCode Aligner3.5.7진행서렬병접，MEGA5.0계산토형피적충내급기여혼위품적충간 K2P거리，구건NJ분자계통수，병분석토형피급기혼위품ITS2서렬이급결구적분자형태학특정。결과：토형피약재ITS2서렬단배형여기기원식물협편서렬일치；토형피급기혼위품적ITS2서렬충간유전거리원대우토형피충내양본적유전거리；NJ수현시토형피양품단독취위일지，약재토형피정위품적ITS2서렬이급결구차이현저。결론：ITS2가이작위토형피급기위품감정적유효DNA조형마서렬。
Pseudolaricis Cortex and its adulterants were identified using DNA barcoding technique in this study. The sequences of ITS2 of Pseudolaricis Cortex and its five adulterants including 37 samples were analyzed. Se-quences were assembled using CodonCode Aligner 3.5.7. K2P distances were calculated and NJ tree was per-formed applying MEGA5.0. The ITS2 secondary structure was predicted using the ITS2 database and website. The results showed that the haplotypes of ITS2 regions of Pseudolaricis Cortex were the same as that of the original plant leaves. The inter-specific K2P distances of ITS2 were obviously higher than the intra-specific ones. Sam-ples of Pseudolaricis Cortex were clustered into a single clade in the NJ tree. The secondary structure of ITS2 of Pseudolaricis Cortex was significantly different from its adulterants. Therefore, ITS2 could powerfully discriminate Pseudolaricis Cortex and its adulterants.