世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2013年
3期
371-380
,共10页
张辉%姚辉%崔丽娜%杜鹤%林喆%高晓晨%郎雪%宋经元%罗焜%石林春%陈士林
張輝%姚輝%崔麗娜%杜鶴%林喆%高曉晨%郎雪%宋經元%囉焜%石林春%陳士林
장휘%요휘%최려나%두학%림철%고효신%랑설%송경원%라혼%석림춘%진사림
动物药材%药典%COI序列%DNA条形码%鉴定
動物藥材%藥典%COI序列%DNA條形碼%鑒定
동물약재%약전%COI서렬%DNA조형마%감정
Animal medical material%Chinese Pharmacopoeia%COI sequences%DNA barcoding%identification
药用动物是我国中药资源的重要组成部分，线粒体 COI基因被公认为动物界中标准的DNA条形码基因，但利用此技术系统研究药用动物或动物药材鉴定的报道不多。本研究选取药典中45个动物药材，涉及基原物种51种，分析样品 COI 序列的种内种间变异情况，barcoding gap，鉴定效率等，考察 COI 序列鉴定动物药材的有效性和准确性。结果表明，种间变异最小值远大于种内变异最大值，barcoding gap 图显示种间变异和种内变异重合较少，鉴定效率较高，除节肢动物门外，在物种水平和属水平上，鉴定效率均为100%，所构建的动物药材及其混伪品的 NJ 树能很好地区分正品来源与其混伪品。因此 COI序列作为 DNA条形码适用于药典中动物药材的鉴定。本研究为COI条形码准确鉴定药典中动物药材提供了分子依据，扩充了 DNA 条形码数据库中药用动物序列，并建立了利用此技术鉴定动物药材的具体技术流程。
藥用動物是我國中藥資源的重要組成部分，線粒體 COI基因被公認為動物界中標準的DNA條形碼基因，但利用此技術繫統研究藥用動物或動物藥材鑒定的報道不多。本研究選取藥典中45箇動物藥材，涉及基原物種51種，分析樣品 COI 序列的種內種間變異情況，barcoding gap，鑒定效率等，攷察 COI 序列鑒定動物藥材的有效性和準確性。結果錶明，種間變異最小值遠大于種內變異最大值，barcoding gap 圖顯示種間變異和種內變異重閤較少，鑒定效率較高，除節肢動物門外，在物種水平和屬水平上，鑒定效率均為100%，所構建的動物藥材及其混偽品的 NJ 樹能很好地區分正品來源與其混偽品。因此 COI序列作為 DNA條形碼適用于藥典中動物藥材的鑒定。本研究為COI條形碼準確鑒定藥典中動物藥材提供瞭分子依據，擴充瞭 DNA 條形碼數據庫中藥用動物序列，併建立瞭利用此技術鑒定動物藥材的具體技術流程。
약용동물시아국중약자원적중요조성부분，선립체 COI기인피공인위동물계중표준적DNA조형마기인，단이용차기술계통연구약용동물혹동물약재감정적보도불다。본연구선취약전중45개동물약재，섭급기원물충51충，분석양품 COI 서렬적충내충간변이정황，barcoding gap，감정효솔등，고찰 COI 서렬감정동물약재적유효성화준학성。결과표명，충간변이최소치원대우충내변이최대치，barcoding gap 도현시충간변이화충내변이중합교소，감정효솔교고，제절지동물문외，재물충수평화속수평상，감정효솔균위100%，소구건적동물약재급기혼위품적 NJ 수능흔호지구분정품래원여기혼위품。인차 COI서렬작위 DNA조형마괄용우약전중동물약재적감정。본연구위COI조형마준학감정약전중동물약재제공료분자의거，확충료 DNA 조형마수거고중약용동물서렬，병건립료이용차기술감정동물약재적구체기술류정。
Medicinal animals are important part of Traditional Chinese medicine resources in China. Cytochrome c oxidase subunit I (COI) was selected as the standard DNA barcoding sequence for animal medical materials. In this study, the 51 animal species from 45 animal medical materials in the Chinese Pharmacopoeia were selected and the intra-specific variation and the inter-specific divergence, the barcoding gap, the identification efficiency of their COI sequences were analyzed. The results showed that the inter-specific divergence is higher than intra-specific distance. The barcoding gap existed between inter-specific sequence divergence and intra-specific dis-tance. The identification efficiencies were 100% both at the genus and species level except the Arthropoda. The cluster dendrogram exhibited that different species distinguished from others. Therefore, COI sequence as a bar-code is suitable to identify the species of animal medical materials in Chinese Pharmacopoeia.
