CAJ | 학술논문

　　丹参为唇形科鼠尾草属多年生落叶开花植物，以根与根茎部位入药，广泛用于多种疾病的治疗。本研究采用生物信息学方法，从丹参基因组框架图序列中发掘出4832个长度超过40 bp的基因组SSR位点。结果表明，二核苷酸重复基元和三核苷酸重复基元是构成丹参基因组SSR位点的主要类型，分别占全部SSR位点总数的37.3%和61.3%。此外，富含A/T碱基的SSR位点，包括AT/TA、AAT/ATT、ATA/TAT、TAA/TTA，分别占全部SSR位点总数的30.5%，21.6%，17.1%，20.4%，在数量上明显高于其他类型。以基因组SSR位点为基础，共设计基因组SSR引物序列1079对。这些引物可用于基因组多样性分析、遗传图谱构建、分子标记筛选等工作，为后期丹参群体遗传学与基因组学研究奠定基础。
　　단삼위진형과서미초속다년생락협개화식물，이근여근경부위입약，엄범용우다충질병적치료。본연구채용생물신식학방법，종단삼기인조광가도서렬중발굴출4832개장도초과40 bp적기인조SSR위점。결과표명，이핵감산중복기원화삼핵감산중복기원시구성단삼기인조SSR위점적주요류형，분별점전부SSR위점총수적37.3%화61.3%。차외，부함A/T감기적SSR위점，포괄AT/TA、AAT/ATT、ATA/TAT、TAA/TTA，분별점전부SSR위점총수적30.5%，21.6%，17.1%，20.4%，재수량상명현고우기타류형。이기인조SSR위점위기출，공설계기인조SSR인물서렬1079대。저사인물가용우기인조다양성분석、유전도보구건、분자표기사선등공작，위후기단삼군체유전학여기인조학연구전정기출。
Salvia miltorrhiza Bge. is a perennial deciduous flowering plant. Its medicinal root and rhizomes part is widely used in the treatment of various diseases. In this study, bioinformatics analysis was performed to identify 4832 genome SSR loci with length longer than or equal to 40 bp from the draft genome assembly of S. miltorrhiza. The re-sults showed that the dinucleotide repeat motifs and trinucleotide repeat motifs constitute the main types of genome SSR loci, accounting for 37.3% and 61.3% respectively. SSR types enriched with A/T bases showed significantly higher abundance than other types, including AT/TA AAT/ATT, ATA/TAT, TAA/TTA, accounting for 30.5%, 21.6%, 17.1%, 20.4% of the total number of SSR loci, respectively. 1079 primer pairs were designed for these genome SSR loci. These primers can be used for genomic diversity analysis, genetic map construction, genetic marker screening. These data could lay the foundation for population genetics and genomics research of S. miltorrhiza.