CAJ | 학술논문

　　甘草酸是中药甘草的主要有效成分，法呢基焦磷酸合酶（FPS）是甘草酸合成途径中关键酶之一。根据甘草转录组数据设计引物，对FPS基因进行克隆，获得甘草FPS基因的全长编码序列。甘草FPS基因编码区全长1029 bp，编码342个氨基酸残基。将甘草FPS基因与GenBank中其他植物FPS基因序列进行比对分析，发现其核酸序列与鹰嘴豆、苜蓿、蒺藜苜蓿、大豆、百脉根的序列相似度最高，分别达到94%、94%、93%、93%、92%。进一步的系统进化分析表明FPS基因具有较高的保守性，其序列进化树与植物分类一致。
　　감초산시중약감초적주요유효성분，법니기초린산합매（FPS）시감초산합성도경중관건매지일。근거감초전록조수거설계인물，대FPS기인진행극륭，획득감초FPS기인적전장편마서렬。감초FPS기인편마구전장1029 bp，편마342개안기산잔기。장감초FPS기인여GenBank중기타식물FPS기인서렬진행비대분석，발현기핵산서렬여응취두、목숙、질려목숙、대두、백맥근적서렬상사도최고，분별체도94%、94%、93%、93%、92%。진일보적계통진화분석표명FPS기인구유교고적보수성，기서렬진화수여식물분류일치。
Glycyrrhizin is the major bioactive compound in Glycyrrhiza uralensis. Farnesyl-diphosphate synthase (FPS) is one of the major enzymes involved in the glycyrrhizin biosynthetic. We cloned the full length of FPS coding sequence based on the transcriptome data of G. uralensis. The FPS gene of G. uralensis (GlyurFPS) is 1029 bp, coding 342 amino acid. The homology between the sequence of its protein and that of the FPS in Cicer arietinum, Medicago sativa, Medicago truncatula, Glycine max, Lotus japonicus was of 94%, 94%, 93%, 93% and 92%, respectively. Plant FPS gene is very conservative, therefore the phylogenetic tree is same with the plant classification consistent.