CAJ | 학술논문

试验旨在研究野菊花多糖对雏鸡血清新城疫抗体水平的影响。将500只7日龄雏鸡滴鼻接种新城疫活疫苗，随机分为5个试验组，每组10个重复，每个重复10只鸡。1~4组（CP-1、CP-5、CP-10、CP-20）雏鸡分别颈部皮下注射1、5、10 mg/mL和20 mg/mL野菊花多糖0.5 mL，每日1次，连续3 d。5组为空白组，用生理盐水进行注射。28日龄，各组雏鸡二次接种新城疫油疫苗，同时按照7日龄时的处理方式给雏鸡注射野菊花多糖和生理盐水。在第1次接种后的0、7、14、21、28、35、42、49、56 d和63 d，每组随机选择20只鸡（每重复2只）进行心脏采血并制备血清，用以分析血清新城疫抗体滴度。结果表明，与对照组相比，第一次接种疫苗14 d后，CP-5、CP-10组和CP-20组雏鸡新城疫抗体滴度均显著提高（P≤0.05），CP-1组雏鸡新城疫抗体滴度在接种后21 d显著提高（P≤0.05）。另外，试验发现随着野菊花多糖剂量的增加，各试验组雏鸡新城疫抗体水平均显著提高（P≤0.05），当注射浓度超过10 mg/mL时，这种促进作用不再随浓度的增加而显著提高（P>0.05）。由此可见，野菊花多糖能够促使雏鸡较早产生特异性抗体并使之维持更长时间，提高疫苗的免疫效果，最佳使用浓度为10 mg/mL。
시험지재연구야국화다당대추계혈청신성역항체수평적영향。장500지7일령추계적비접충신성역활역묘，수궤분위5개시험조，매조10개중복，매개중복10지계。1~4조（CP-1、CP-5、CP-10、CP-20）추계분별경부피하주사1、5、10 mg/mL화20 mg/mL야국화다당0.5 mL，매일1차，련속3 d。5조위공백조，용생리염수진행주사。28일령，각조추계이차접충신성역유역묘，동시안조7일령시적처리방식급추계주사야국화다당화생리염수。재제1차접충후적0、7、14、21、28、35、42、49、56 d화63 d，매조수궤선택20지계（매중복2지）진행심장채혈병제비혈청，용이분석혈청신성역항체적도。결과표명，여대조조상비，제일차접충역묘14 d후，CP-5、CP-10조화CP-20조추계신성역항체적도균현저제고（P≤0.05），CP-1조추계신성역항체적도재접충후21 d현저제고（P≤0.05）。령외，시험발현수착야국화다당제량적증가，각시험조추계신성역항체수평균현저제고（P≤0.05），당주사농도초과10 mg/mL시，저충촉진작용불재수농도적증가이현저제고（P>0.05）。유차가견，야국화다당능구촉사추계교조산생특이성항체병사지유지경장시간，제고역묘적면역효과，최가사용농도위10 mg/mL。
The study was conducted to evaluate the effects of Chrysanthemum indicuml polysaccharides (CP) on the production of the serum newcastle disease (ND) antibody in vaccinated chickens.500 7-day old chickens intranasally in-oculated ND vaccine,were randomly divided into 5 groups with 10 replicates of 10 each.1 to 4 group (CP-1,CP-5,CP-10,CP-20)chickens were subcutaneously injected 0.5 mL CP with the concentrations of 1,5,10 mg/mL and 20 mg/mL, respectively,once a day for 3 consecutive days.The control group was injected with saline instead of CP.When 28-day old, all chickens were inoculated ND oil vaccine and injected the CP and saline in the same way of 7-day old. 0,7,14,21,28,35,42,49,56,63 day after the first inoculation,20 chickens( 2 birds per replicate from )were randomly se-lected from each group to draw the heart blood and prepare the serum.The results showed that:the ND HI antibody titers of CP-5,CP-10 and CP-20 groups were significantly improved compared with control group,after 14 d (P≤0.05),and which of CP-1 group increased significantly after 21 d (P ≤ 0.05).In addition,the ND antibody titers were significantly increased with the dose increasings until 10 mg/mL (CP-10) of the Chrysanthemum indicum polysaccharide groups(P ≤0.05).The results indicated that CP could promote specific antibody production earlier and maintain it longer,and thus im-prove the immune effect of the vaccine.The optimum dose of CP was 10 mg/mL.