国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2013年
5期
399-402
,共4页
双侧颈动脉狭窄%家兔%低血压%神经元特异性烯醇化酶%S100β
雙側頸動脈狹窄%傢兔%低血壓%神經元特異性烯醇化酶%S100β
쌍측경동맥협착%가토%저혈압%신경원특이성희순화매%S100β
Bilateral carotid stenosis%Rabbits%Hypotension%Neuron-specific enolase%S100β
目的 观察不同干预所致低血压时双侧颈动脉中度狭窄家兔模型血浆神经元特异性烯醇化酶(neuron-specific enolase,NSE)及S100β浓度的变化,探讨围术期低血压对颈动脉狭窄兔脑组织的损伤作用.方法 选择成年家兔建立双侧颈动脉中度狭窄家兔模型,采用完全随机法分为4组,除对照组外,其他3组分别采用放血,硝酸甘油,丙泊酚使血压降低20％～30％并维持20 min后恢复血压,分别于恢复血压后7.5、24h取血液标本,测定血清中NSE及S100β蛋白,将脑损伤的指标做比较. 结果 与对照组比较3个降压组的血液标本中NSE及S100β在各个观测时间点的值均升高,差异有统计学意义(P＜0.05).丙泊酚组在降压7.5 h的NSE及S100β的表达值分别为(1 487±19) μg/L和(592±10) μg/L,与放血组、硝酸甘油组比较,各时间点NSE蛋白表达指标的升高值较小,差异有统计学意义(P＜0.05);S 100β蛋白的表达较其余两个实验组低,差异有统计学意义(P＜0.05). 结论 对于双侧颈动脉中度狭窄兔模型,放血、硝酸甘油及麻醉药物引起的血压降低20％～30％均引起NSE及S100β蛋白的表达升高,其中丙泊酚组的升高程度相对较低,可能与丙泊酚的脑保护作用有关.
目的 觀察不同榦預所緻低血壓時雙側頸動脈中度狹窄傢兔模型血漿神經元特異性烯醇化酶(neuron-specific enolase,NSE)及S100β濃度的變化,探討圍術期低血壓對頸動脈狹窄兔腦組織的損傷作用.方法 選擇成年傢兔建立雙側頸動脈中度狹窄傢兔模型,採用完全隨機法分為4組,除對照組外,其他3組分彆採用放血,硝痠甘油,丙泊酚使血壓降低20％～30％併維持20 min後恢複血壓,分彆于恢複血壓後7.5、24h取血液標本,測定血清中NSE及S100β蛋白,將腦損傷的指標做比較. 結果 與對照組比較3箇降壓組的血液標本中NSE及S100β在各箇觀測時間點的值均升高,差異有統計學意義(P＜0.05).丙泊酚組在降壓7.5 h的NSE及S100β的錶達值分彆為(1 487±19) μg/L和(592±10) μg/L,與放血組、硝痠甘油組比較,各時間點NSE蛋白錶達指標的升高值較小,差異有統計學意義(P＜0.05);S 100β蛋白的錶達較其餘兩箇實驗組低,差異有統計學意義(P＜0.05). 結論 對于雙側頸動脈中度狹窄兔模型,放血、硝痠甘油及痳醉藥物引起的血壓降低20％～30％均引起NSE及S100β蛋白的錶達升高,其中丙泊酚組的升高程度相對較低,可能與丙泊酚的腦保護作用有關.
목적 관찰불동간예소치저혈압시쌍측경동맥중도협착가토모형혈장신경원특이성희순화매(neuron-specific enolase,NSE)급S100β농도적변화,탐토위술기저혈압대경동맥협착토뇌조직적손상작용.방법 선택성년가토건립쌍측경동맥중도협착가토모형,채용완전수궤법분위4조,제대조조외,기타3조분별채용방혈,초산감유,병박분사혈압강저20％～30％병유지20 min후회복혈압,분별우회복혈압후7.5、24h취혈액표본,측정혈청중NSE급S100β단백,장뇌손상적지표주비교. 결과 여대조조비교3개강압조적혈액표본중NSE급S100β재각개관측시간점적치균승고,차이유통계학의의(P＜0.05).병박분조재강압7.5 h적NSE급S100β적표체치분별위(1 487±19) μg/L화(592±10) μg/L,여방혈조、초산감유조비교,각시간점NSE단백표체지표적승고치교소,차이유통계학의의(P＜0.05);S 100β단백적표체교기여량개실험조저,차이유통계학의의(P＜0.05). 결론 대우쌍측경동맥중도협착토모형,방혈、초산감유급마취약물인기적혈압강저20％～30％균인기NSE급S100β단백적표체승고,기중병박분조적승고정도상대교저,가능여병박분적뇌보호작용유관.
Objective To observe the changes of neuron-specific enolase (NSE)and S100β concentration in plasma of the bilateral carotid moderate stenosis rabbits following hypotension induced by different interventions,and to explore the injury effect of perioperative hypotension on carotid stenosis rabbit brain.Methods Choose the adult rabbit to establish bilateral carotid moderate stenosis rabbit model.The rabbits were randomly divided into four groups.in addition to the control group,the other three groups rabbits all decreased blood pressure by 20％-30％ induced by bloodletting,nitroglycerin and propofol,and maintain for 20 min,than restore blood pressure,Took blood samples,after blood pressure restoration 7.5,24 h respectively and measured NSE and S100β protein in serum,then compared with the extent of brain damage.Results Compared with the blood samples of the control group,NSE and S100β values in each observation time point all increased in three hypotension groups,and there wree significant difference (P＜0.05).NSE and S 100β values were (1 486.51 ±19.22) μg/L and (592.13 ± 10.09) μg/L respectively in propofol group after decreasing blood pressure 7.5 h,NSE protein expression index increased a little than the bloodletting group,nitroglycerin group at each time point,There were significant difference among these groups (P＜0.05).S100β protein expression was decreased when compared with the other two experimental groups at 7.5 h,and there was,significant difference (P＜0.05).Conclusions For the moderate bilateral carotid stenosis rabbit model,the expressions of NSE and S100β protein are both increased when blood pressure is reduced by 20％-30％ induced by bloodletting,nitroglycerin and narcotic drugs.The increase degree in propofol group is relatively light,which may be related to the cerebral protective effects of propofol.
