CAJ | 학술논문

为研究单个PC12细胞中多巴胺的含量,以碳纤维纳米电极为工作电极,自行组装了微流控芯片安培柱末检测系统,设计了一种微流控芯片电泳单细胞进样和溶膜的控制方式,仅需一路高压控制即可实现单细胞进样和溶膜操作.结果表明：采用该系统实现了4种儿茶酚胺类物质的基线分离,通过控制适当的细胞密度及液池的液面高度差,实现了单个细胞连续快速进样、溶膜和分析,得出单个PC12细胞中多巴胺含量为0.80±0.33 fmol（n=5）.
위연구단개PC12세포중다파알적함량,이탄섬유납미전겁위공작전겁,자행조장료미류공심편안배주말검측계통,설계료일충미류공심편전영단세포진양화용막적공제방식,부수일로고압공제즉가실현단세포진양화용막조작.결과표명：채용해계통실현료4충인다분알류물질적기선분리,통과공제괄당적세포밀도급액지적액면고도차,실현료단개세포련속쾌속진양、용막화분석,득출단개PC12세포중다파알함량위0.80±0.33 fmol（n=5）.
A microchip electrophoresis system with end-channel carbon fiber nanoelectrode amperometric detector was developed to study on the content of DA in single phochromocytoma（PC12） cell.An operation mode was designed to achieve single cell injection and lysis in chip-CE with only one high-voltage power supply.The experimental results indicate that four catecholamines were baseline-separated and determined with this system.The cell density and liquid height of the reservoirs were accommodated for single cell loading,docking and analysis.The microchip CE system has been successfully applied to the determination of dopamine（DA） in single cultured rat PC12 cell and the average content of DA in single PC12 cell is 0.80±0.33（n=5） fmol.