人参研究
人參研究
인삼연구
RENSHEN YANJIU
2012年
1期
13-14
,共2页
曲正义%赵景辉%刘宏群%姚春林%王英平
麯正義%趙景輝%劉宏群%姚春林%王英平
곡정의%조경휘%류굉군%요춘림%왕영평
保鲜人参%索氏提取%人参总皂苷%比色法%含量测定
保鮮人參%索氏提取%人參總皂苷%比色法%含量測定
보선인삼%색씨제취%인삼총조감%비색법%함량측정
Fresh-keeping ginseng%Soxhlet extraction%Total ginsenoside%Colorimetry%Determination
目的采用索氏热回流提取保鲜人参中的人参总皂苷。方法香草醛一硫酸比色法测定保鲜人参中人参总皂苷的含量,检测波长544nm。结果索氏热回流提取可将保鲜人参中的人参总皂苷提取完全,在20-120μg(r=0.9995)范围内线性关系良好,平均回收率为107.86%,BSD=3.49%(n=5)。测得样品中总皂苷含量为3.91%,RSD=I.84%(11=3)。结论该方法操作简便,稳定性和重现性较好,可用于保鲜人参中人参总皂苷的含量测定。
目的採用索氏熱迴流提取保鮮人參中的人參總皂苷。方法香草醛一硫痠比色法測定保鮮人參中人參總皂苷的含量,檢測波長544nm。結果索氏熱迴流提取可將保鮮人參中的人參總皂苷提取完全,在20-120μg(r=0.9995)範圍內線性關繫良好,平均迴收率為107.86%,BSD=3.49%(n=5)。測得樣品中總皂苷含量為3.91%,RSD=I.84%(11=3)。結論該方法操作簡便,穩定性和重現性較好,可用于保鮮人參中人參總皂苷的含量測定。
목적채용색씨열회류제취보선인삼중적인삼총조감。방법향초철일류산비색법측정보선인삼중인삼총조감적함량,검측파장544nm。결과색씨열회류제취가장보선인삼중적인삼총조감제취완전,재20-120μg(r=0.9995)범위내선성관계량호,평균회수솔위107.86%,BSD=3.49%(n=5)。측득양품중총조감함량위3.91%,RSD=I.84%(11=3)。결론해방법조작간편,은정성화중현성교호,가용우보선인삼중인삼총조감적함량측정。
The fresh-keeping ginseng was extracted by soxhlet extraction with hot methanol reflux method, And then,The colorimetry method analysis was employed,The detected wavelength was 544 nm. Results indicated th;tt A good linear relationship was observed in the range of 20-120μg (r=0.9995)and averagerecovery rate was 107.86% with a relative standard deviation of 3.49%(n=5). The content of total ginsenoside in the samples from Ji'an city,Ji lin region was 3.91%,with 1.84% of RSD (n=3).