辣椒杂志
辣椒雜誌
랄초잡지
JOURNAL OF CHINA CAPSICUM
2012年
1期
33-37
,共5页
周倩%叶新华%赵尊练%史联联%武国平%谢振华%郭建伟
週倩%葉新華%趙尊練%史聯聯%武國平%謝振華%郭建偉
주천%협신화%조존련%사련련%무국평%사진화%곽건위
线辣椒%病毒病%ELISA检测%试纸检测
線辣椒%病毒病%ELISA檢測%試紙檢測
선랄초%병독병%ELISA검측%시지검측
linear pepper%virus%ELISA%test strip
病毒病是陕西关中地区线辣椒的主要病害。关于该地区线辣椒病毒病毒源鉴定已经有过文献报道。但是,随着品种的更新、栽培环境的演化以及病原的分化等,有必要对其毒源进行定期地检测,以便为病毒病的防控提供依据。该研究在陕西省关中西部线辣椒主产区(千阳县、凤翔县、周至县)根据田间宏观症状随机采集当地主栽品种和部分育种优系的叶样,利用ELISA检测蚕豆萎蔫病毒(BBWV)等6种病毒,同时利用检测试纸对黄瓜花叶病毒(CMV)进行现场检测。ELISA结果表明,检测的55份样品均携带1种以上病毒,病毒侵染率高达100%。有2个样品携带所检测的全部6种病毒。在检测的6种病毒中,BBWV,PMMV、CMV、ToMV、TMV和PVY的检出率分别为100%、81.8%、76.4%、43.6%、41.8%和23.6%。CMV试纸检测的18个样品全部携带CMV病毒。
病毒病是陝西關中地區線辣椒的主要病害。關于該地區線辣椒病毒病毒源鑒定已經有過文獻報道。但是,隨著品種的更新、栽培環境的縯化以及病原的分化等,有必要對其毒源進行定期地檢測,以便為病毒病的防控提供依據。該研究在陝西省關中西部線辣椒主產區(韆暘縣、鳳翔縣、週至縣)根據田間宏觀癥狀隨機採集噹地主栽品種和部分育種優繫的葉樣,利用ELISA檢測蠶豆萎蔫病毒(BBWV)等6種病毒,同時利用檢測試紙對黃瓜花葉病毒(CMV)進行現場檢測。ELISA結果錶明,檢測的55份樣品均攜帶1種以上病毒,病毒侵染率高達100%。有2箇樣品攜帶所檢測的全部6種病毒。在檢測的6種病毒中,BBWV,PMMV、CMV、ToMV、TMV和PVY的檢齣率分彆為100%、81.8%、76.4%、43.6%、41.8%和23.6%。CMV試紙檢測的18箇樣品全部攜帶CMV病毒。
병독병시협서관중지구선랄초적주요병해。관우해지구선랄초병독병독원감정이경유과문헌보도。단시,수착품충적경신、재배배경적연화이급병원적분화등,유필요대기독원진행정기지검측,이편위병독병적방공제공의거。해연구재합서성관중서부선랄초주산구(천양현、봉상현、주지현)근거전간굉관증상수궤채집당지주재품충화부분육충우계적협양,이용ELISA검측잠두위언병독(BBWV)등6충병독,동시이용검측시지대황과화협병독(CMV)진행현장검측。ELISA결과표명,검측적55빈양품균휴대1충이상병독,병독침염솔고체100%。유2개양품휴대소검측적전부6충병독。재검측적6충병독중,BBWV,PMMV、CMV、ToMV、TMV화PVY적검출솔분별위100%、81.8%、76.4%、43.6%、41.8%화23.6%。CMV시지검측적18개양품전부휴대CMV병독。
The virus diseases are major ones of linear pepper in Guanzhong region of Shaanxi. Although linear pepper virus identification in this region has been reported, a regular detection is necessary to control the virus diseases because of new varieties, environmental factors and pathogenic differentiation. In this study, some leaf samples were collected randomly based on virus macroscopic symptoms in the main producing areas of linear pepper. The 6 viruses (BBWV, PMMV, CMV, ToMV, TMV and PVY) were detected by means of ELISA. At the same time, the CMV was detected with CMV test strip. The results of ELISA showed that all of the 55 samples detected carried one kind of virus or more, the infection rate was 100%. Among the 6 viruses, the infection rateof BBWV, PMMV, CMV, ToMV, TMV and PVY was 100%, 81.8%, 76.4%, 43.6%, 41.8% and 23.6%, respectively. The results of test strip showed that all the 18 samples detected carried the CMV.