CAJ | 학술논문

用碱性蛋白酶解法制备花生多肽，将得到的花生肽酶解液通过超滤的方法进行分离得到不同分子量段的多肽，对其进行抗氧化活性的研究得到抗氧化活性最强的分子量段的多肽。将花生短肽酶解液稀释并分别通过5KD、3KD的卷式纤维膜进行超滤得到三个分子量段的花生短肽。比较各分子量段的多肽对二苯代苦味酰基（DPPH）自由基的清除率、抗亚油酸氧化能力、羟自由基的清除率及还原力。结果表明：花生肽具有一定的抗氧化能力，且3KD以下的多肽抗氧化能力最强，3-5KD分子量段的多肽次之，大于5KD分子量的多肽表现出较弱的抗氧化能力。
용감성단백매해법제비화생다태，장득도적화생태매해액통과초려적방법진행분리득도불동분자량단적다태，대기진행항양화활성적연구득도항양화활성최강적분자량단적다태。장화생단태매해액희석병분별통과5KD、3KD적권식섬유막진행초려득도삼개분자량단적화생단태。비교각분자량단적다태대이분대고미선기（DPPH）자유기적청제솔、항아유산양화능력、간자유기적청제솔급환원력。결과표명：화생태구유일정적항양화능력，차3KD이하적다태항양화능력최강，3-5KD분자량단적다태차지，대우5KD분자량적다태표현출교약적항양화능력。
The peanut peptide was obtained with hydrolysis defatted peanut flakes, which was separated by different ultrafiltration membranes to three different size peptide. And then the reducing power, hydroxyl radical scavenging, antioxidant and DPPH radical scavenging were determined with different concentration and molecular weight of peanut peptide. The experimental results showed that the peanut peptide had relatively strong antioxidant activity, and with the different molecular weight of peanut peptide, they showed different antioxidant activity. The molecular weight less than 3KD showed the strongest antioxidant activity, 3-5KD was the next, and the molecular weight more than 5KD showed the weakest antioxidant activity.