草食家畜
草食傢畜
초식가축
GRASS-FEEDING LIVESTOCK
2012年
1期
45-49
,共5页
张淑琴%张读朴%黄炯%钟旗%吴冬玲
張淑琴%張讀樸%黃炯%鐘旂%吳鼕玲
장숙금%장독박%황형%종기%오동령
布氏杆菌病活疫苗%冻干保护剂%培养基%活菌计数%残余水分
佈氏桿菌病活疫苗%凍榦保護劑%培養基%活菌計數%殘餘水分
포씨간균병활역묘%동간보호제%배양기%활균계수%잔여수분
Brucellosis vaccine%freeze protection agent%medium%viable count
通过对布氏杆菌病疫苗的生产工艺方面进行了部分优化:在原明胶蔗糖保护剂的基础上添加改良剂10%脱脂奶粉后,残余水分比原来有所下降,基本上解决了残余水分偏高的现象;结晶现象、不脱壁现象已完全解决;同时活菌计数的活茵数提高了3%。结果表明新的布氏杆菌复合培养基不受培养基原材料的质量因素影响,且疫苗的生产工艺稳定,易于保存,半成品的活菌数提高了28%,疫苗冻干后的活菌数提高了39%。
通過對佈氏桿菌病疫苗的生產工藝方麵進行瞭部分優化:在原明膠蔗糖保護劑的基礎上添加改良劑10%脫脂奶粉後,殘餘水分比原來有所下降,基本上解決瞭殘餘水分偏高的現象;結晶現象、不脫壁現象已完全解決;同時活菌計數的活茵數提高瞭3%。結果錶明新的佈氏桿菌複閤培養基不受培養基原材料的質量因素影響,且疫苗的生產工藝穩定,易于保存,半成品的活菌數提高瞭28%,疫苗凍榦後的活菌數提高瞭39%。
통과대포씨간균병역묘적생산공예방면진행료부분우화:재원명효자당보호제적기출상첨가개량제10%탈지내분후,잔여수분비원래유소하강,기본상해결료잔여수분편고적현상;결정현상、불탈벽현상이완전해결;동시활균계수적활인수제고료3%。결과표명신적포씨간균복합배양기불수배양기원재료적질량인소영향,차역묘적생산공예은정,역우보존,반성품적활균수제고료28%,역묘동간후적활균수제고료39%。
The production process of Brucellosis vaccine was partinally optimized by our company. Through adding the modifier 10% skim milk on the basis of the original gelatin sucrose protective agent, residual moisture content declined relative to the original and the residual water being on the high side phenomenon had been resolved basically and Crystallization and off the wall phenomenon had been resolved completely.At the same time living bacteria rate increased by 3% through the viable bacteria counting. The results show that the new brucellosis composite medium is not affected from the culture medium raw material quality factors and vaccine production process is stability and easy storage, living bacteria rate of semi-finished products and freeze-dried vaccine were improved respectively by 28% and 39%.