生命科学仪器
生命科學儀器
생명과학의기
LIFE SCIENCES INSTRUMENT
2012年
2期
19-21
,共3页
离子色谱%小麦粉%溴酸盐%Ag/H柱%RP(C18)柱
離子色譜%小麥粉%溴痠鹽%Ag/H柱%RP(C18)柱
리자색보%소맥분%추산염%Ag/H주%RP(C18)주
Ion chromatography%wheat flour%bromate%Ag/H column%RP(C18)column
建立离子色谱法测定小麦粉及其小麦粉制品中溴酸盐的测定方法。采用的色谱柱为戴安的AS19(250×4.6mm),淋洗液(流动相)为KOH溶液。用纯水提取样品中溴酸根离子(BrO3-),经RP(C18)柱除去样品中得有机物,经Ag/H柱除去样品提取液中干扰氯离子(Cl-),采用离子交换色谱-电导检测器测定,外标法定量,经测试溴酸盐在0.05~2.0μg/mL范围内,线性良好,线性方程为y=1.435x-0.031,相关系数R=0.9998,添加浓度在1.60mg/kg~4.80mg/kg范围内,平均回收率在97.5%~98.4%之间。
建立離子色譜法測定小麥粉及其小麥粉製品中溴痠鹽的測定方法。採用的色譜柱為戴安的AS19(250×4.6mm),淋洗液(流動相)為KOH溶液。用純水提取樣品中溴痠根離子(BrO3-),經RP(C18)柱除去樣品中得有機物,經Ag/H柱除去樣品提取液中榦擾氯離子(Cl-),採用離子交換色譜-電導檢測器測定,外標法定量,經測試溴痠鹽在0.05~2.0μg/mL範圍內,線性良好,線性方程為y=1.435x-0.031,相關繫數R=0.9998,添加濃度在1.60mg/kg~4.80mg/kg範圍內,平均迴收率在97.5%~98.4%之間。
건립리자색보법측정소맥분급기소맥분제품중추산염적측정방법。채용적색보주위대안적AS19(250×4.6mm),림세액(류동상)위KOH용액。용순수제취양품중추산근리자(BrO3-),경RP(C18)주제거양품중득유궤물,경Ag/H주제거양품제취액중간우록리자(Cl-),채용리자교환색보-전도검측기측정,외표법정량,경측시추산염재0.05~2.0μg/mL범위내,선성량호,선성방정위y=1.435x-0.031,상관계수R=0.9998,첨가농도재1.60mg/kg~4.80mg/kg범위내,평균회수솔재97.5%~98.4%지간。
A method for determination of bromate in wheat flour and wheat flour products by Ion chromatography method is reported.The chromatographic column for DIONEX AS19(250mm×4.0mm),eluent(mobile phase) for KOH solution,Conductivity detector,extraced by pure water samples of bromate ion(BrO-3),RP(C18) column was used to remove the organic matter,the Ag/H column to remove sample extraction liquid disturbance in the chloride ion(Cl-),using ion chromatography conductivity detector for the determination,quantified by external standard method.Linear ranges of lutein was 0.05~2.0μg/mL,the linear equation was y=1.435x-0.031,relevant index R2=0.9998;Concentration in the 1.60mg/kg~4.80mg/kg range,the average recovery rate were 92.8~103.5%.