生命科学仪器
生命科學儀器
생명과학의기
LIFE SCIENCES INSTRUMENT
2012年
2期
3-5
,共3页
高效液相色谱%维生素A%维生素E%奶粉
高效液相色譜%維生素A%維生素E%奶粉
고효액상색보%유생소A%유생소E%내분
HPLC%Vitamin A%Vitamin E%Milk Powder
目的：建立用高效液相色谱法同时测定奶粉中维生素A、E含量的方法。方法：奶粉样品经过皂化处理后,直接取上清液进样到高效液相色谱仪中,紫外双波长检测。结果：维生素A、E的浓度线性范围分别为0.25～1.25μg/mL、2.50～12.50μg/mL,最低检出浓度分别为0.511μg/100g、13.3μg/100g;相对标准偏差为1.0%~5.7%,回收率为98.0%~102.2%。结论：该法简单、快速、准确,满足检测需要,适合奶粉中维生素A和E的检测。
目的：建立用高效液相色譜法同時測定奶粉中維生素A、E含量的方法。方法：奶粉樣品經過皂化處理後,直接取上清液進樣到高效液相色譜儀中,紫外雙波長檢測。結果：維生素A、E的濃度線性範圍分彆為0.25～1.25μg/mL、2.50～12.50μg/mL,最低檢齣濃度分彆為0.511μg/100g、13.3μg/100g;相對標準偏差為1.0%~5.7%,迴收率為98.0%~102.2%。結論：該法簡單、快速、準確,滿足檢測需要,適閤奶粉中維生素A和E的檢測。
목적：건립용고효액상색보법동시측정내분중유생소A、E함량적방법。방법：내분양품경과조화처리후,직접취상청액진양도고효액상색보의중,자외쌍파장검측。결과：유생소A、E적농도선성범위분별위0.25～1.25μg/mL、2.50～12.50μg/mL,최저검출농도분별위0.511μg/100g、13.3μg/100g;상대표준편차위1.0%~5.7%,회수솔위98.0%~102.2%。결론：해법간단、쾌속、준학,만족검측수요,괄합내분중유생소A화E적검측。
[Objective] To establish a HPLC method for simultaneously detecting vitamin A and E in milk powder.[Method] The processed supernatant of the milk powder after saponification was injected into the HPLC and detected with dual ultraviolet wavelength.[Results] The linear range of Vitamin A and E were 0.25～1.25μg/mL and 2.50～12.50μg/mL.The recoveries of vitamin A and E were 98.0%-102.2%,the RSD was 1.0%-5.7%,the minimal detection concentration was 0.511 μg/100g and 13.3 μg/100g respectively.[Conclusion] The method is simple,accurate and precise and is suitable for determining vitamin A and E in milk powder.
