CAJ | 학술논문

根据成人疾病发育起源的假说,早期发育中的不利影响会导致永久的生理和代谢改变,导致成年后疾病风险增长,建立了大鼠PCOS动物模型,为PCOS的发病机制及治疗方法的研究提供工具.实验采用方法为：将孕鼠分为雄激素组和正常对照组,每组5只,于孕鼠第10d起,雄激素组每天每只注射二氢睾酮1.2mg,对照组注射同体积的生理盐水,待2组仔鼠（正常组13只,二氢睾酮组16只）出生后生长至14~16周,处死,取卵巢观察2组仔鼠卵巢形态,取静脉血测血性激素变化、空腹血糖、胰岛素水平及血脂变化.研究结果表明：雄激素组仔鼠卵巢囊性改变、高雄激素血症、血糖增高以及性周期不规则,与人的多囊卵巢综合症部分特征相似.
근거성인질병발육기원적가설,조기발육중적불리영향회도치영구적생리화대사개변,도치성년후질병풍험증장,건립료대서PCOS동물모형,위PCOS적발병궤제급치료방법적연구제공공구.실험채용방법위：장잉서분위웅격소조화정상대조조,매조5지,우잉서제10d기,웅격소조매천매지주사이경고동1.2mg,대조조주사동체적적생리염수,대2조자서（정상조13지,이경고동조16지）출생후생장지14~16주,처사,취란소관찰2조자서란소형태,취정맥혈측혈성격소변화、공복혈당、이도소수평급혈지변화.연구결과표명：웅격소조자서란소낭성개변、고웅격소혈증、혈당증고이급성주기불규칙,여인적다낭란소종합증부분특정상사.
According to the disease development hypothesis,adverse effects in early stage lead to permanent changes in physiology and metabolism,resulting in increased risk of suffering diseases in adulthood.In this report,we build a rat PCOS model to gain a deeper understanding of the pathogenesis of PCOS and to evaluate treatment alternatives.Pregnant rats were randomly divided into two experimental groups（n=13,DHT： n=16）,on the 15th day of pregnancy,1.2 mg DHT was injected daily with identical volume physiologic saline.The two Pups were raised to 14-16 weeks followed by life extermination,at which time the ovaries were extracted for an array of observations including ovarian morphology,blood gonadal hormone concentration,glucose concentration.The insulin concentrations were tested,and the lipid metabolism was measured.It is found that the DHT rats show the increase in the number of cystic follicles,hyperandrogenic status,hyperglycemia and irregular cycles.The proposed models are expected to make some contributions to the studies of PCOS.