CAJ | 학술논문

以黑龙江省东北林业大学帽儿山实验林场的3种典型木材腐朽菌为试验材料，采用TRAP分子标记的手段，针对5种木质素与纤雏素相关酶基因LiP、MnP、Lac、CBHⅡ、CDH设计引物，初步分析这些编码基因的多态性。试验确定了3种木腐菌TRAP分子标记的反应体系和反应程序。经过对32对引物进行筛选试验，共确定11对引物，包括3对标记MnP编码基因的引物、3对标记Lac编码基因的引物、3对标记CBH编码基因的引物，以及2对标记CDH编码基因的引物。结果表明：3种木腐菌的TRAP．PCR标记共产生了265条条带，其中多态性条带206条，占总条带的77．74％，多态性最高为100％，最低为60％，证明了TRAP分子标记可以应用于木腐菌的遗传分析。
이흑룡강성동북임업대학모인산실험림장적3충전형목재부후균위시험재료，채용TRAP분자표기적수단，침대5충목질소여섬추소상관매기인LiP、MnP、Lac、CBHⅡ、CDH설계인물，초보분석저사편마기인적다태성。시험학정료3충목부균TRAP분자표기적반응체계화반응정서。경과대32대인물진행사선시험，공학정11대인물，포괄3대표기MnP편마기인적인물、3대표기Lac편마기인적인물、3대표기CBH편마기인적인물，이급2대표기CDH편마기인적인물。결과표명：3충목부균적TRAP．PCR표기공산생료265조조대，기중다태성조대206조，점총조대적77．74％，다태성최고위100％，최저위60％，증명료TRAP분자표기가이응용우목부균적유전분석。
Collecting three typical wood-rotting fungi from Maoer Mountain Forest Farm of Northeast Forestry University as the experimental materials, a preliminary study on polymorphism of the encoding genes of five lignocellulose enzyme, including lip, mnp, lac, cbh II and cdh, have been done. At the same time, the reaction system and process of TRAP-PCR of three wood-rotting fungi have been optimized firstly, and then 11 pair primers were selected from 32 pair primers, including 3 pair primers of MnP encoding gene, 3 pair of Lae encoding gene, 3 pair of CBH encoding gene, as well as 2 pair of CDH encoding gene. The result shows that three wood-rotting fungi generated 265 bands by TRAP-PCR, among which 206 bands are polymorphic bands with 77.74% in potymorphic rate ranging from 60% in minimum and 100% in maximum. This experiment proved the possibility of genetic analysis of wood- rotting fungus by using TRAP marker.