生物质化学工程
生物質化學工程
생물질화학공정
BIOMASS CHEMICAL ENGINEERING
2012年
2期
25-29
,共5页
南方红豆杉%紫杉醇%分布%HPLC
南方紅豆杉%紫杉醇%分佈%HPLC
남방홍두삼%자삼순%분포%HPLC
Taxus chinensis var mairei%paclitaxel%distribution%HPLC
采用高效液相色谱法对野生和人工栽培南方红豆杉约20年生植株各器官、组织中紫杉醇含量进行检测分析后发现,紫杉醇在南方红豆杉植株中具有极显著向下、向根际积累分布的规律。野生和载培红豆杉,在器官水平,从上部枝叶到根部须根,紫杉醇的积累分别为0.007 61%～0.100 54%和0.015 25%～0.061 75%（干重,质量分数,下同）;在组织水平,从形态学上部到根部,木质组织中紫杉醇积累分别为0.010 95%～0.044 76%和0～0.017 58%,周皮组织中紫杉醇的积累量也具有从形态学上部到下部增高的规律,在主枝皮中的含量分别为0.013 32%和0.006 77%,在根皮中的含量分别为0.086 33%和0.059 74%。野生南方红豆杉中紫杉醇产量比人工栽培的要高。分析过程中紫杉醇在21～105 mg/L范围内线性关系良好,r2为0.999,平均加标回收率为85.78%,相对标准偏差为1.03%。
採用高效液相色譜法對野生和人工栽培南方紅豆杉約20年生植株各器官、組織中紫杉醇含量進行檢測分析後髮現,紫杉醇在南方紅豆杉植株中具有極顯著嚮下、嚮根際積纍分佈的規律。野生和載培紅豆杉,在器官水平,從上部枝葉到根部鬚根,紫杉醇的積纍分彆為0.007 61%～0.100 54%和0.015 25%～0.061 75%（榦重,質量分數,下同）;在組織水平,從形態學上部到根部,木質組織中紫杉醇積纍分彆為0.010 95%～0.044 76%和0～0.017 58%,週皮組織中紫杉醇的積纍量也具有從形態學上部到下部增高的規律,在主枝皮中的含量分彆為0.013 32%和0.006 77%,在根皮中的含量分彆為0.086 33%和0.059 74%。野生南方紅豆杉中紫杉醇產量比人工栽培的要高。分析過程中紫杉醇在21～105 mg/L範圍內線性關繫良好,r2為0.999,平均加標迴收率為85.78%,相對標準偏差為1.03%。
채용고효액상색보법대야생화인공재배남방홍두삼약20년생식주각기관、조직중자삼순함량진행검측분석후발현,자삼순재남방홍두삼식주중구유겁현저향하、향근제적루분포적규률。야생화재배홍두삼,재기관수평,종상부지협도근부수근,자삼순적적루분별위0.007 61%～0.100 54%화0.015 25%～0.061 75%（간중,질량분수,하동）;재조직수평,종형태학상부도근부,목질조직중자삼순적루분별위0.010 95%～0.044 76%화0～0.017 58%,주피조직중자삼순적적루량야구유종형태학상부도하부증고적규률,재주지피중적함량분별위0.013 32%화0.006 77%,재근피중적함량분별위0.086 33%화0.059 74%。야생남방홍두삼중자삼순산량비인공재배적요고。분석과정중자삼순재21～105 mg/L범위내선성관계량호,r2위0.999,평균가표회수솔위85.78%,상대표준편차위1.03%。
Accumulation distribution of paclitaxel in entire plant of Taxus chinensis var.mairei was investigated.The content of paclitaxel in organs and tissues of T.chinensis var mairei that was wild or cultivatied about 20 years old was analyzed by HPLC.The results show that the paclitaxel in the plant is downward and has rhizosphere accumulation significantly.At the organ level,the contents of paclitaxel from branch leaves to fibrous root are 0.007 61 %-0.100 54 %（dry weight,wild） and 0.015 25 %-0.061 75 %（dry weight,cultivation） respectively.In the tissue level,the contents of paclitaxel in xylem tissue from morpholo-gical upper part to root are 0.010 95 %-0.044 76 %（dry weight,wild） and 0-0.017 58 %（dry weight,cultivation）.The accumulation of paclitaxel in skin tissue increase from morphological upper part to lower part.The contents of paclitaxel in main branch bark are 0.013 32 %（dry weight,wild） and 0.006 77 %（dry weight,cultivation）,and in root bark are 0.08633%（dry weight,wild） and 0.059 74 %（dry weight,cultivation） respectively.The output of paclitaxel in wild taxus chinensis is higher than that in cultured taxus chinensis.The distribution of paclitaxel was linear at its contents 21-105 mg/L（r2=0.999）.The average recovery was 85.78%,and the RSD was 1.03%.
