CAJ | 학술논문

目的研究外源性S100a8和S100a9与结直肠癌侵袭转移的关系。方法 Real-time PCR检测巨噬细胞RAW264.7及结肠癌细胞CT26.WT中S100a8和S100a9的基因表达水平；ELISA检测RAW264.7及CT26.WT细胞分泌至胞外的S100a8和S100a9的蛋白含量。划痕实验和Transwel 实验检测外源性S100a8和S100a9蛋白对结肠癌细胞侵袭转移的影响。结果S100a8和S100a9在巨噬细胞中的表达明显高于结肠癌细胞（=0.0126；=0.03）；外源性S100a8和S100a9均能促进结肠癌细胞发生侵袭和转移（=0.0128；=0.0011）。结论结肠癌肿瘤微环境中S100a8和S100a9主要在巨噬细胞中高表达和分泌，并发挥促结肠癌侵袭转移的作用，有可能成为治疗结肠癌进展的新靶点。
목적연구외원성S100a8화S100a9여결직장암침습전이적관계。방법 Real-time PCR검측거서세포RAW264.7급결장암세포CT26.WT중S100a8화S100a9적기인표체수평；ELISA검측RAW264.7급CT26.WT세포분비지포외적S100a8화S100a9적단백함량。화흔실험화Transwel 실험검측외원성S100a8화S100a9단백대결장암세포침습전이적영향。결과S100a8화S100a9재거서세포중적표체명현고우결장암세포（=0.0126；=0.03）；외원성S100a8화S100a9균능촉진결장암세포발생침습화전이（=0.0128；=0.0011）。결론결장암종류미배경중S100a8화S100a9주요재거서세포중고표체화분비，병발휘촉결장암침습전이적작용，유가능성위치료결장암진전적신파점。
Objective To study the relationships among the exogenous S100a8/S100a9 and metastasis in colon carcinoma. Methods The gene expression of S100a8 and S100a9 were detected by Real-time PCR in murine macrophage cellline RAW264.7 cells and murine colon carcinoma cellline CT26.WT .The quantification of S100a8 and S100a9 secretion in supernatants of RAW264.7 cells and CT26.WT cells were detected by ELISA. In addition, the ef ects of exogenous S100a8 and S100a9 on the invasion and metastasis of colon carcinoma cells were performed by wound healing and cellinvasion assay. Results The S100a8 and S100a9 expression levels in macrophage cellRAW264.7 were significantly higher than in colon carcinoma cell CT26.WT ( =0.0126;=0.03 respectively). Furthermore, both of S100a8 and S100a9 have the ability to promote the invasion and metastasis of colon carcinoma cells significantly ( =0.0128;=0.0011 respectively). Conclusion The results suggested that S100a8 and S100a9 are mainly expressed and secreted in macrophage cells among the tumor environment, which might be valuable in the invasion and metastasis of colon carcinoma cells and might become new therapeutic targets of colon carcinoma.