广东畜牧兽医科技
廣東畜牧獸醫科技
엄동축목수의과기
GUANDONG JOURNAL OF ANIMAL AND VETERINARY SCIENCE
2012年
2期
23-26
,共4页
苏伟桐%钟植文%赵金旺%杨傲冰%刘镇明%张毓金
囌偉桐%鐘植文%趙金旺%楊傲冰%劉鎮明%張毓金
소위동%종식문%조금왕%양오빙%류진명%장육금
鸭黄病毒%产蛋下降%分离%鉴定
鴨黃病毒%產蛋下降%分離%鑒定
압황병독%산단하강%분리%감정
Duck flavivirus%Sharp decrease in egg production%Isolation%Identification
2010年4月以来,我国部分地区陆续发生以产蛋鸭产蛋急剧下降为特点的疾病。从广东省江门地区某鸭场的发病鸭群采集样品,接种鸭胚后分离到一株病毒,并命名为JM株,经RT-PCR检测为黄病毒核酸阳性。序列测定后分析显示,该分离株JM株与黄病毒属Tembusu病毒的对应序列相似性最高,其中与Tembusu病毒JS804株相似性最高达98%。结果表明,JM株为黄病毒,与该病鸭产蛋急剧下降有关。
2010年4月以來,我國部分地區陸續髮生以產蛋鴨產蛋急劇下降為特點的疾病。從廣東省江門地區某鴨場的髮病鴨群採集樣品,接種鴨胚後分離到一株病毒,併命名為JM株,經RT-PCR檢測為黃病毒覈痠暘性。序列測定後分析顯示,該分離株JM株與黃病毒屬Tembusu病毒的對應序列相似性最高,其中與Tembusu病毒JS804株相似性最高達98%。結果錶明,JM株為黃病毒,與該病鴨產蛋急劇下降有關。
2010년4월이래,아국부분지구륙속발생이산단압산단급극하강위특점적질병。종광동성강문지구모압장적발병압군채집양품,접충압배후분리도일주병독,병명명위JM주,경RT-PCR검측위황병독핵산양성。서렬측정후분석현시,해분리주JM주여황병독속Tembusu병독적대응서렬상사성최고,기중여Tembusu병독JS804주상사성최고체98%。결과표명,JM주위황병독,여해병압산단급극하강유관。
Since April 2010, a new duck infectious disease characterized by sharp decrease in egg production emerged in some areas in China. Some samples were collected from the diseased ducks in Jiangmen city of Guangdong province. Virus JM was isolated from one sample through duck embryo inoculation and was detected positive by duck flavivirus RT-PCR method. Sequence analysis based on NS5 sequence showed that strain JM was most closely related to flavivirus strain Tembusu and had great similarity (98%) with strain JS804. The results showed that strain JM belonged to flavivirus and might be the reason for the sharp decrease in egg production.