CAJ | 학술논문

目的本研究旨在检测GPR56及其mRNA在胶质瘤组织中的表达状态，分析GPR56表达程度与胶质瘤级别的相关性。方法收集38例胶质瘤患者标本，以6例脑外伤患者切除组织为对照组，分别以免疫组化SP法及RT-PCR半定量检测法检测GPR56及其mRNA在胶质瘤组织中的表达水平，并对结果进行统计分析。结果研究结果显示，GPR56在胶质母细胞瘤表达的阳性率为90%(18/20)，星形胶质细胞瘤Ⅰ～Ⅲ级阳性率为100%(18/18)，正常脑组织中阳性率为0；38例脑胶质瘤中GPR56 mRNA的表达为84%(32/38)；且GPR56 mRNA的表达在低级别与高级别胶质瘤之间无显著性差异(P>0.05)。结论 GPR56在胶质瘤中高表达，且胶质瘤标本中GPR56 mRNA的表达比较稳定，可作为一种标记物。
목적본연구지재검측GPR56급기mRNA재효질류조직중적표체상태，분석GPR56표체정도여효질류급별적상관성。방법수집38례효질류환자표본，이6례뇌외상환자절제조직위대조조，분별이면역조화SP법급RT-PCR반정량검측법검측GPR56급기mRNA재효질류조직중적표체수평，병대결과진행통계분석。결과연구결과현시，GPR56재효질모세포류표체적양성솔위90%(18/20)，성형효질세포류Ⅰ～Ⅲ급양성솔위100%(18/18)，정상뇌조직중양성솔위0；38례뇌효질류중GPR56 mRNA적표체위84%(32/38)；차GPR56 mRNA적표체재저급별여고급별효질류지간무현저성차이(P>0.05)。결론 GPR56재효질류중고표체，차효질류표본중GPR56 mRNA적표체비교은정，가작위일충표기물。
Objects Aim to measure levels of GPR56 and mRNA in glioma, and analysis its relations with grades of glioma.Methods 38 specimens were col ected in our hospital, comparing with 6 slices of traumatic brain injury. Then immunohistochemistry and RT-PCR were conducted to measure levels of GPR56 and mRNA in glioma.Results Immuohistochemistry results showed GPR56 had 90% positive expression in 20 glioblastoma multiforme specimens, had 100% positive expression in 18 gradeⅠ~Ⅲ astrocytoma specimens, and no positive expression in normal specimens. RT-PCR results showed that GPR56 mRNA was expressed in 84% (32/38) glioma tissues. But it had no signifficant difference between the tumor grade and GPR56 mRNA expression (P>0.05).Conclusion GPR56 and GPR56 mRNAhad a high and stable expression in glioma. It might be a kind of biomarker.