神经损伤与功能重建
神經損傷與功能重建
신경손상여공능중건
NEURAL INJURY AND FUNCTIONAL RECONSTRUCTION
2013年
3期
177-180
,共4页
何佳%丁凤菲%刘敏珍%王伟
何佳%丁鳳菲%劉敏珍%王偉
하가%정봉비%류민진%왕위
VEGF/VEGFR%缺血半暗带%脑内皮细胞
VEGF/VEGFR%缺血半暗帶%腦內皮細胞
VEGF/VEGFR%결혈반암대%뇌내피세포
VEGF/VEGFR%ischemic penumbra%brain endothelial cells
目的:研究VEGF/VEGFR在脑缺血再灌注损伤中的神经保护作用。方法:SD大鼠108只,随机分为假手术组、对照组与VEGFR抗体组,每组36只,制备大脑中动脉缺血再灌注损伤(MCAO)模型,假手术组不造成缺血。VEGFR抗体组在造模前20 min侧脑室注射VEGF抗体,假手术组、对照组侧脑室注射等量生理盐水。比较3组在造模后1、3、7 d Bederson评分、VEGFR及缺血半暗带VEGFR2的变化。结果:VEGFR抗体组的Bederson评分在第1、3天高于对照组(P约0.05),VEGFR的表达在第1、3天低于对照组(P约0.01),但较假手术组高;VEGFR抗体组VEGFR2表达在第1、3天低于对照组(P约0.01)。结论:脑缺血再灌注损伤时,VEGF活化刺激VEGFR表达上调,促进血管内皮细胞增殖,加速新生血管形成。
目的:研究VEGF/VEGFR在腦缺血再灌註損傷中的神經保護作用。方法:SD大鼠108隻,隨機分為假手術組、對照組與VEGFR抗體組,每組36隻,製備大腦中動脈缺血再灌註損傷(MCAO)模型,假手術組不造成缺血。VEGFR抗體組在造模前20 min側腦室註射VEGF抗體,假手術組、對照組側腦室註射等量生理鹽水。比較3組在造模後1、3、7 d Bederson評分、VEGFR及缺血半暗帶VEGFR2的變化。結果:VEGFR抗體組的Bederson評分在第1、3天高于對照組(P約0.05),VEGFR的錶達在第1、3天低于對照組(P約0.01),但較假手術組高;VEGFR抗體組VEGFR2錶達在第1、3天低于對照組(P約0.01)。結論:腦缺血再灌註損傷時,VEGF活化刺激VEGFR錶達上調,促進血管內皮細胞增殖,加速新生血管形成。
목적:연구VEGF/VEGFR재뇌결혈재관주손상중적신경보호작용。방법:SD대서108지,수궤분위가수술조、대조조여VEGFR항체조,매조36지,제비대뇌중동맥결혈재관주손상(MCAO)모형,가수술조불조성결혈。VEGFR항체조재조모전20 min측뇌실주사VEGF항체,가수술조、대조조측뇌실주사등량생리염수。비교3조재조모후1、3、7 d Bederson평분、VEGFR급결혈반암대VEGFR2적변화。결과:VEGFR항체조적Bederson평분재제1、3천고우대조조(P약0.05),VEGFR적표체재제1、3천저우대조조(P약0.01),단교가수술조고;VEGFR항체조VEGFR2표체재제1、3천저우대조조(P약0.01)。결론:뇌결혈재관주손상시,VEGF활화자격VEGFR표체상조,촉진혈관내피세포증식,가속신생혈관형성。
Objective:To research the neuroprotective effect of VEGF/VEGFR after cerebral ischemia reperfu-sion injury. Methods:One hundred and eight SD rats were randomly divided into sham group, control group, and VEGFR antibody group, with 36 rats in each group. To establish middle cerebral artery occlusion(MCAO) models, while the sham group had no ischemia. At 20 min Before MCAO modeling, VEGFR antibody group received an intracerebroventricular injection of VEGF antibody, while sham group and control group only re-ceived an intracerebroventricular injection of same amount of normal saline. Then to compare the changes of Bederson score, VEGFR and VEGFR2 expression of 3 groups at 1, 3, 7 days after MCAO. Results:The Beder-son score of VEGFR antibody group was higher than control group at 1, 3 d (P<0.05). The VEGFR expression in VEGFR antibody group was lower than control group at 1, 3 d(P<0.01), while higher than sham group. The VEGFR2 expression in VEGFR antibody group was lower than control group at 1, 3 d (P<0.01). Conclusion:After cerebral ischemia reperfusion injury, VEGF’s activation can promote the expression of VEGFR, which promote proliferation of brain vascular endothelial cells and accelerate the formation of new blood vessels.