水生生物学报
水生生物學報
수생생물학보
ACTA HYDROBIOLOGICA SINICA
2014年
1期
58-67
,共10页
温度%盐度%渗透压%皮质醇%Na+-K+-ATP酶%抗氧化酶%脂质过氧化%褐牙鲆幼鱼
溫度%鹽度%滲透壓%皮質醇%Na+-K+-ATP酶%抗氧化酶%脂質過氧化%褐牙鲆幼魚
온도%염도%삼투압%피질순%Na+-K+-ATP매%항양화매%지질과양화%갈아평유어
Temperature%Salinity%Osmotic pressure%Cortisol%Na+-K+-ATPase%Antioxidant enzyme%Lipid peroxida-tion%Juvenile Japanese flounder
采用双因素析因实验设计方法,研究了温度(20℃、24℃、28℃)和盐度(10‰、30‰)急性应激对褐牙鲆(Paralichthys olivaceus)幼鱼渗透生理和抗氧化水平的影响。结果表明：盐度和温度变化对各实验组1d和6d时褐牙鲆幼鱼血浆皮质醇含量均无显著性差异。在高温低盐(28℃、10‰)环境中1d 时渗透压显著高于其他各实验组,6d时无显著性差异。牙鲆幼鱼在28℃环境中1d时鳃Na+-K+-ATP酶活性显著高于20℃和24℃；6d时,温度和盐度对牙鲆幼鱼鳃Na+-K+-ATP酶活性有显著交互影响作用。1d时,随着温度的升高或盐度的降低牙鲆幼鱼肝脏超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性呈现上升趋势,并且高温低盐(28℃、盐度10‰)组褐牙鲆幼鱼肝脏丙二醛(MDA)含量显著高于其他各组；在3个实验温度下,10‰环境中牙鲆幼鱼肝脏脂质过氧化物(LPO)的含量高于30‰。在6d时,各实验组间肝脏SOD、CAT活性及MDA含量无显著性差异。因此,褐牙鲆能够耐受温度20-28℃和低至盐度10‰的环境条件,应激早期温度和盐度的变化可引起褐牙鲆幼鱼渗透生理和抗氧化水平的变化,高温低盐对褐牙鲆幼鱼抗氧化水平的影响最大,至6d 可基本恢复稳定。
採用雙因素析因實驗設計方法,研究瞭溫度(20℃、24℃、28℃)和鹽度(10‰、30‰)急性應激對褐牙鲆(Paralichthys olivaceus)幼魚滲透生理和抗氧化水平的影響。結果錶明：鹽度和溫度變化對各實驗組1d和6d時褐牙鲆幼魚血漿皮質醇含量均無顯著性差異。在高溫低鹽(28℃、10‰)環境中1d 時滲透壓顯著高于其他各實驗組,6d時無顯著性差異。牙鲆幼魚在28℃環境中1d時鰓Na+-K+-ATP酶活性顯著高于20℃和24℃；6d時,溫度和鹽度對牙鲆幼魚鰓Na+-K+-ATP酶活性有顯著交互影響作用。1d時,隨著溫度的升高或鹽度的降低牙鲆幼魚肝髒超氧化物歧化酶(SOD)和過氧化氫酶(CAT)活性呈現上升趨勢,併且高溫低鹽(28℃、鹽度10‰)組褐牙鲆幼魚肝髒丙二醛(MDA)含量顯著高于其他各組；在3箇實驗溫度下,10‰環境中牙鲆幼魚肝髒脂質過氧化物(LPO)的含量高于30‰。在6d時,各實驗組間肝髒SOD、CAT活性及MDA含量無顯著性差異。因此,褐牙鲆能夠耐受溫度20-28℃和低至鹽度10‰的環境條件,應激早期溫度和鹽度的變化可引起褐牙鲆幼魚滲透生理和抗氧化水平的變化,高溫低鹽對褐牙鲆幼魚抗氧化水平的影響最大,至6d 可基本恢複穩定。
채용쌍인소석인실험설계방법,연구료온도(20℃、24℃、28℃)화염도(10‰、30‰)급성응격대갈아평(Paralichthys olivaceus)유어삼투생리화항양화수평적영향。결과표명：염도화온도변화대각실험조1d화6d시갈아평유어혈장피질순함량균무현저성차이。재고온저염(28℃、10‰)배경중1d 시삼투압현저고우기타각실험조,6d시무현저성차이。아평유어재28℃배경중1d시새Na+-K+-ATP매활성현저고우20℃화24℃；6d시,온도화염도대아평유어새Na+-K+-ATP매활성유현저교호영향작용。1d시,수착온도적승고혹염도적강저아평유어간장초양화물기화매(SOD)화과양화경매(CAT)활성정현상승추세,병차고온저염(28℃、염도10‰)조갈아평유어간장병이철(MDA)함량현저고우기타각조；재3개실험온도하,10‰배경중아평유어간장지질과양화물(LPO)적함량고우30‰。재6d시,각실험조간간장SOD、CAT활성급MDA함량무현저성차이。인차,갈아평능구내수온도20-28℃화저지염도10‰적배경조건,응격조기온도화염도적변화가인기갈아평유어삼투생리화항양화수평적변화,고온저염대갈아평유어항양화수평적영향최대,지6d 가기본회복은정。
A factorial experiment was designed to evaluate the combined effects of temperature (20℃, 24℃ and 28℃) and salinity (10‰ and 30‰) on the osmotic pressure and antioxidant responses in the juvenile Japanese flounder (Paralichthys olivaceus). The results showed that the temperature and salinity caused no significant differences in the plasma cortisol of the juvenile Japanese flounder exposed in the experimental conditions for either 1 day (1 day after exposure, 1DAE) or 6 DAE. High temperature and low salinity (28℃, salinity10‰) significantly enhanced osmotic pressure level of the juvenileP. olivaceus in 1 DAE, but no significant difference in 6 DAE was observed. The Na+-K+-ATPase activity in the gill at 28℃ was significantly higher than that in 19℃ and 24℃ in 1 DAE, and the interactive effect between temperature and salinity on Na+-K+-ATPase activity was highly remarkable in 6 DAE. High temperature and low salinity increased SOD and CAT activity of juvenileP. olivaceus in 1 DAE, and the MDA content in the treatment (28℃, salinity 10‰) was significantly higher than the other treatments. The LPO content of juvenileP. olivaceus in salinity 10‰ was higher than that in the salinity 30‰ in 1 DAE at each temperature. There were no significant differences in liver SOD,CAT activity or MDA content in 1 DAE. Therefore,as euryhaline fish, P. olivaceus has very strong adaptability to environmental changes which allows them to tolerate conditions as high as 28℃ in temperature and as low as 10‰ in salinity. The changes of temperature and salinity may initially result in alterations of physiological activities and antioxidant responses, especially under high temperature and low salinity; and the physiological functions of flounders most likely recover to normal conditions after 6 days.
