CAJ | 학술논문

研究常见饲料原料中胆碱的生物学效价,为渔用配合饲料配制提供基础数据。试验选取团头鲂[平均体重(3.5±0.1) g]990尾,以酪蛋白、明胶等原料配制4个氯化胆碱添加水平(0、1030、1230、1430 mg/kg)的纯合饲料,并在1030 mg/kg的基础上分别添加7种常见饲料原料(鱼粉、豆粕、菜粕、棉粕、次粉、麸皮、米糠),使得各组配合饲料中胆碱含量为1230 mg/kg,共11组,每组3重复,饲养8周。根据肝脏胆碱沉积量和试验鱼胆碱摄食量,评定团头鲂幼鱼对不同浓度的胆碱利用率和这7种原料中胆碱的生物学效价。结果表明：在4个纯和饲料中,肝脏胆碱沉积量和增重率随着饲料胆碱水平从0到1230 mg/kg饲料的增加而显著上升(P<0.05),并在饲料胆碱水平从1230到1430 mg/kg饲料的增加而差异不显著(P>0.05)；在同一胆碱水平(1230 mg/kg)的条件下,原料组的增长率均高于对照组；团头鲂幼鱼对鱼粉、豆粕、菜粕、棉粕、次粉、麸皮和米糠的胆碱生物学效价分别为87.42%、112.54%、76.84%、98.00%、95.91%、43.88%、91.5%。分析可知,团头鲂生产饲料中尚需要额外添加氯化胆碱方能满足其对胆碱的需要,实际添加量与饲料所使用的原料有关。
연구상견사료원료중담감적생물학효개,위어용배합사료배제제공기출수거。시험선취단두방[평균체중(3.5±0.1) g]990미,이락단백、명효등원료배제4개록화담감첨가수평(0、1030、1230、1430 mg/kg)적순합사료,병재1030 mg/kg적기출상분별첨가7충상견사료원료(어분、두박、채박、면박、차분、부피、미강),사득각조배합사료중담감함량위1230 mg/kg,공11조,매조3중복,사양8주。근거간장담감침적량화시험어담감섭식량,평정단두방유어대불동농도적담감이용솔화저7충원료중담감적생물학효개。결과표명：재4개순화사료중,간장담감침적량화증중솔수착사료담감수평종0도1230 mg/kg사료적증가이현저상승(P<0.05),병재사료담감수평종1230도1430 mg/kg사료적증가이차이불현저(P>0.05)；재동일담감수평(1230 mg/kg)적조건하,원료조적증장솔균고우대조조；단두방유어대어분、두박、채박、면박、차분、부피화미강적담감생물학효개분별위87.42%、112.54%、76.84%、98.00%、95.91%、43.88%、91.5%。분석가지,단두방생산사료중상수요액외첨가록화담감방능만족기대담감적수요,실제첨가량여사료소사용적원료유관。
To evaluate dietary choline availability values in juvenile blunt snout breamMegalobrama amblycephala, an 8-week feeding trial was conducted with seven common feed ingredients. Choline chloride was fortified to the basal diet that contained 310 g/kg diet from vitamin-free casein and gelatin to formulate four purified diets containing 0, 1030, 1230 and 1430 mg/kg choline, respectively. These four purified diets were used to generate a standard curve to evaluate choline availability in seven diets containing the following common feed ingredients: fish meal (FM), soybean meal (SBM), rapeseed meal (RSM), cottonseed meal (CSM), wheat middling (WM), wheat bran (WB) and rice bran (RB). The choline in seven diets was 1230 mg/kg including 1030 mg/kg choline chloride. Three groups ofMegalobrama am-blycephala with initial average weight (3.5±0.1) g were fed randomly with each diet in a flow-through system. Our re-sults indicated that weight gain and liver choline concentration were significantly increased for higher dietary choline levels (P <0.05) in four purified diets. Based on liver choline data, choline availability values were 87.42%, 112.54%, 76.84%, 98%, 95.91%, 43.88% and 91.5% in FM, SBM, RSM, CSM, WM, WB and RB, respectively. These findings demonstrate that choline chloride should be fortified in feeds, which depends on ingredients.