CAJ | 학술논문

在pH为7．2的Tris-HCI缓冲溶液中，脂蛋白（a）抗体胶乳液与脂蛋白（a）发生免疫反应并在胶乳E聚集，导致体系的共振散射强度增强，在波长为580mm处有较强的共振散射峰。在选定条件下，脂蛋白（a）浓度在0．06～1．40μg·mL。范围内与580am处的散射强度呈良好的线性关系，其回归方程为A1Rs=325．6p-17．8，相关系数为0．996，检出限为0．0063μg·mL^-1。用于血清中脂蛋白a测定，仪器简单，操作简便，选择性好，结果满意。
재pH위7．2적Tris-HCI완충용액중，지단백（a）항체효유액여지단백（a）발생면역반응병재효유E취집，도치체계적공진산사강도증강，재파장위580mm처유교강적공진산사봉。재선정조건하，지단백（a）농도재0．06～1．40μg·mL。범위내여580am처적산사강도정량호적선성관계，기회귀방정위A1Rs=325．6p-17．8，상관계수위0．996，검출한위0．0063μg·mL^-1。용우혈청중지단백a측정，의기간단，조작간편，선택성호，결과만의。
In pH 7.2 Tris-HCl buffer solution, anti-human lipoprotein（a） latex combine with Lipoprotein（a） and exhibit a stronger resonance scattering at 580 nm. The lipoprotein（a） concentration p in the range of 0.06-1.40μg·mL-t is linear to the resonance scattering intensity at 580 nm.The regress equation is AIRs =325.6p-17.8, the coefficient is 0.996 andits detection limitis achieved at 0.0063μg·mL^-1lipoprotein（a）. The proposed method was applied to the determination of lipoprotein（a） in plasma, with simplicity, sensitivity, good selectivity, and satisfactory results.