CAJ | 학술논문

以稻草秸杆为纤维素原料，用2％氢氧化钠对其进行预处理，选用纤维素酶作为催化剂对纤维素进行酶法水解，采用DINS法测定纤维索水解液还原糖的含量，并计算其糖化率。探讨了纤维素酶水解稻草秸秆纤维素的反应机理，研究了纤维素酶的添加量、反应温度、pH、反应时间、振荡等因素对稻草纤维素酶水解的影响。实验结果表明，当固液比为1：30、纤维素酶添加量为40mg、50℃、pH4．8、振荡反应12h，纤维素酶解液的糖化率可达40％。
이도초갈간위섬유소원료，용2％경양화납대기진행예처리，선용섬유소매작위최화제대섬유소진행매법수해，채용DINS법측정섬유색수해액환원당적함량，병계산기당화솔。탐토료섬유소매수해도초갈간섬유소적반응궤리，연구료섬유소매적첨가량、반응온도、pH、반응시간、진탕등인소대도초섬유소매수해적영향。실험결과표명，당고액비위1：30、섬유소매첨가량위40mg、50℃、pH4．8、진탕반응12h，섬유소매해액적당화솔가체40％。
Rice straw cellulose was hydrolyzed by cellulase, using 2 % NaOH as pretreatment agent, content of reducing sugar was mensurated using the DNS method, and then the saccharification rate was calculated.The hydrolytic mechanism of straw cellulose was researched, the effect factors such as the adding amount of cellulase, reaction temperature, pH, reaction time, vibration were studied. The experimental results showed that hydrolysis rate could be as high as 40 % as the adding amount of Cellulase was 40 mg, 50 ~C, pH was 4.8, vibration reaction persist with 12h.