CAJ | 학술논문

以橙皮苷为标准品，采用紫外分光光度法（直接法）和高效液相色谱（HPLC）测定橙皮苷含量，并对测定条件进行探讨，比较两种方法测定橙皮苷的准确度。实验结果表明，紫外分光光度法测得的橙皮苷纯度数据比高效液相色谱数据高，HPLC法精确，分光光度法简单快速，均能得到令人满意的结果。
이등피감위표준품，채용자외분광광도법（직접법）화고효액상색보（HPLC）측정등피감함량，병대측정조건진행탐토，비교량충방법측정등피감적준학도。실험결과표명，자외분광광도법측득적등피감순도수거비고효액상색보수거고，HPLC법정학，분광광도법간단쾌속，균능득도령인만의적결과。
Hesperidin as standard sample, the content of hesperidin was determined with UV - Vis spectrophotometry method and high performance liquid chromoma to graphy （HPLC）, and the determine conditions were discussed. Mean- while, compared with these two determination methods, the accuracies were compared. The results showed that the purity of hesperidin analyzed by UV - Vis spectrophotometry method was marginally higher than that of HPLC. UV - Vis spectro- photometry method was simple and convenient, while HPLC can get high accuracy result.